Heterologous Expression of the Secale cereal Thaumatin-Like Protein in Transgenic Canola Plants Enhances Resistance to Stem Rot Disease
Authors: not saved
Abstract:
Canola (Brassica napus L.) is an important oilseed crop. A serious problem in cultivation of this crop andyield loss, are due to fungal disease stem rot caused by Sclerotinia sclerotiorum. The pathogenesis-related(PR) proteins have the potential for enhancing resistance against fungal pathogen. Thaumatin-like proteins(TLPs) have been shown to have antifungal activity on various fungal pathogens. In this study, the tlp geneisolated from cereal rye (Secale cereal L.) was introduced into canola plants. The amplified DNA fragment(about 500 bp) was analyzed and confirmed by restriction pattern and cloned into pUC19 and designated aspUCNG1. Comparison of the cloned fragment with the DNA sequence indicated that this gene contains nointron. The tlp gene was predicted to encode a protein of 173 amino acids with an estimated molecular massof 17.7 kDa. The deduced amino acid sequence of TLP showed a significant sequence identity with TLPfrom S.cereal and other plants. We used a transgenic over-expression approach in order to investigate antifungal activity of expressed TLP on Sclerotinia sclerotiorum. TLP was overexpressed under the CaMV35Sconstitutive promoter in (Brassica napus, R line Hyola 308). Transformation of cotyledonary petioles wasachieved via Agrobacterium tumefaciens LBA4404. The insertion of transgene was verified by the polymerasechain reaction (PCR) and genomic DNA dot blotting. Antifungal activity was detected in transgeniccanola lines using detached leaf assay. The size of lesions induced by S. sclerotiorum in the leaves oftransgenic canola was significantly retarded when compared to that detected in non-transgenic plants.
similar resources
Agrobacterium-Mediated Transformation of the Oryza sativa Thaumatin-Like Protein to Canola (R Line Hyola308) for Enhancing Resistance to Sclerotinia sclerotiorum
Background: Canola is an agro-economically oilseed crop. Yield loss due to fungal disease of stem rot caused by Sclerotinia sclerotiorum is a serious problem in canola cultivation. Thaumatin-like proteins are large groups of the pathogenesis-related proteins which provide resistance to the fungal infection in response to invading pathogens and play a key role in plant defense s...
full textExpression Pattern of the Synthetic Pathogen-Inducible Promoter (SynP-FF) in the Transgenic Canola in Response to Sclerotinia sclerotiorum
Sclerotinia sclerotiorum is a phytopathogenic fungus which causes serious yield losses in canola. A pathogen inducible-promoter can facilitate the production of Sclerotinia-resistant transgenic canola plants. Inthis study, the “gain of function approach” was adopted for the construction of a pathogen-inducible promoter.The synthetic promoter technique was used, which involved the in...
full textTransgenic approaches to microbial disease resistance in crop plants.
Recent progress in the genetic dissection of plant disease resistance signaling pathways has opened a number of new avenues towards engineering pathogen resistance in crops. Genes controlling race-specific and broad-spectrum resistance responses have been cloned, and novel induced resistance pathways have been identified in model and crop systems. Advances continue to be made in identification ...
full textImproved shoot regeneration protocol for canola explants and pre-assessment of salinity tolerance in canola transgenic plants
Regeneration of explants plays a significant role in plant transformation. Explant type, hormonal concentration, and pre-culturing period are important in transformation efficiency. To get an efficient transformation of canola and optimize regeneration conditions, different explants along with different culture media were studied. Four canola varieties were used to evaluate regeneration ability...
full textHeterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187
Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...
full textConstitutive expression of the poplar WRKY transcription factor PtoWRKY60 enhances resistance to Dothiorella gregaria Sacc. in transgenic plants.
WRKY proteins are involved in various physiological processes in plants, especially in coping with diverse biotic and abiotic stresses. However, limited information is available on the roles of specific WRKY transcription factors in poplar defense. In this study, we reported the characterization of PtoWRKY60, a Group IIa WRKY member, from Populus tomentosa Carr. The gene expression profile of P...
full textMy Resources
Journal title
volume 10 issue 2
pages 87- 95
publication date 2012-04-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023