Halotolerant Ability and α-Amylase Activity of Some Saltwater Fungal Isolates
Authors
Abstract:
Four halotolerant fungal isolates originating from the saltwater Lake Urmia in Iran were selected during a screening program for salt resistance and α-amylase activity. The isolates were identified based on sequencing the ITS region and a part of the β-tubulin gene, as Penicillium chrysogenum (isolate U1; CBS 132820), Fusarium incarnatum (isolate U2; CBS 132821), and Penicillium polonicum (isolate U3; CBS 132822, and isolate U4; CBS 132823). The growth of these isolates was determined by measuring the colony diameter and mycelia dry weight in Sabouraud dextrose agar and yeast nitrogen base medium supplemented with NaCl, KCl, and LiCl. Isolate U4 showed a growth up in 15% NaCl and U1 was the only isolate that could grow in 20% KCl. None of the strains grew in a media containing LiCl. The salt supplemented medium did not increase the size of colony diameter in all isolates (p > 0.05). The ability of the selected isolates for amylase production was quantitatively tested and showed that P. polonicum isolate U4 was the most potent producer of amylase with a yield of 260.9 U/L after 60 h, whereas P. polonicum isolate U3 was the lowest one with a production level of 97.9 U/L after 48 h. P. polonicum isolate U4 could be a suitable candidate for production of amylase on an industrial scale after optimization.
similar resources
Halotolerant Ability and α-Amylase Activity of Some Saltwater Fungal Isolates
Four halotolerant fungal isolates originating from the saltwater Lake Urmia in Iran were selected during a screening program for salt resistance and α-amylase activity. The isolates were identified based on sequencing the ITS region and a part of the β-tubulin gene, as Penicillium chrysogenum (isolate U1; CBS 132820), Fusarium incarnatum (isolate U2; CBS 132821), and Penicillium polonicum (isol...
full texthalotolerant ability and α-amylase activity of some saltwater fungal isolates
four halotolerant fungal isolates originating from the saltwater lake urmia in iran were selected during a screening program for salt resistance and α-amylase activity. the isolates were identified based on sequencing the its region and a part of the β-tubulin gene, as penicillium chrysogenum (isolate u1; cbs 132820), fusarium incarnatum (isolate u2; cbs 132821), and penicillium polonicum (isol...
full textComparison of α-amylase activity in some sprouting Nigerian cereals
The yield and characteristics of Alpha-amylase obtained from some sprouting Nigeria cereals (maize, acha, rice and sorghum) were compared using standard procedures. The result indicated maximum α-amylase yield was attained from 72-120 hours of sprouting cereals). Of all the sprouted cereals, acha gave rise to the highest amylase yield (16.6 ± 0.02x10 and the least was exhibited by maize (11.0±0...
full textPotential Anti-obesity Effects of some Medicinal Herb: In vitro α-Amylase, α-Glucosidase and Lipase Inhibitory Activity
In this study, in vitro inhibitory activity of methanolic and chloroform extracts of some medicinal plants including C. wightii, T. ammi, N. sativa, C. arabica, L. usitatissimum, C. cyminum, and R. Graveolens was evaluated toward α-glucosidase, α-amylase, and lipase comparing with acarbose and orlistat as the standard inhibitors. Our results revealed that both methanolic and chloroform extracts...
full textHighly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68
An alkaliphilic and highly thermostable α-amylase producing Bacillus sp. was isolated from Van soda lake. Enzyme synthesis occurred at temperatures between 25°C and 40°C. Analysis of the enzyme by SDS-PAGE revealed a single band which was estimated to be 66 kDa. The enzyme was active in a broad temperature range, between 20°C and 90°C, with an optimum at 50°C; and maximum activity was at pH 10....
full textMy Resources
Journal title
volume 12 issue Supplement
pages 113- 119
publication date 2013-03-12
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023