Gene Expression and Pulmonary Toxicity of Chitosan-graft-Polyethylenimine as Aerosol Gene Carrier

Authors

  • Chang Gyu Woo Nanotechnology and Thermal Processing Laboratory, School of Mechanical and Aerospace Engineering, Seoul 151-742, Korea.
  • Chong-Su Cho Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea.
  • Hu-Lin Jiang 1- Laboratory of Toxicology, College of Veterinary Medicine, Seoul 151-742, Korea. 2- School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
  • Jung-Taek Kwon Laboratory of Toxicology, College of Veterinary Medicine, Seoul 151-742, Korea.
  • Mansoo Choi Nanotechnology and Thermal Processing Laboratory, School of Mechanical and Aerospace Engineering, Seoul 151-742, Korea.
  • Myung-Haing Cho 1- Laboratory of Toxicology, College of Veterinary Medicine, Seoul 151-742, Korea. 2- Department of Nanofusion Technology, Graduate School of Convergence Science and Technology, Seoul National University, Suwon 443-270, Korea. 3- Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Korea.
  • Yeon-Soo Kim Indang Institute of Molecular Biology, Inje University, Seoul 100-032, Korea.
Abstract:

Chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer has been used for theimprovement of low transfection efficiency of chitosan. The present study aims to test thepulmonary toxicity and efficiency of CHI-g-PEI as an aerosol gene carrier. Mice were exposedto aerosol containing green-fluorescent protein (GFP)-polyethylenimine (PEI) or GFP-CHIg-PEI complexes for 30 min during the development of our nose-only exposure chamber(NOEC) system. CHI-g-PEI-mediated aerosol delivery demonstrated 15.65% enhancementof the fluorescence intensity. Compared to PEI, CHI-g-PEI showed no significant pulmonarytoxicity. In summary, using CHI-g-PEI is safe and shows high transfection in aerosol genedelivery to animals, and enhanced efficiency was achieved through our aerosol gene deliverysystem. Therefore, CHI-g-PEI and this system would be applicable to future study for aerosolgene therapy.

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Journal title

volume 12  issue 2

pages  281- 286

publication date 2013-06-09

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