Frequency of fimH, papC, and aer in E. coli Strains Isolated from Patients with Urinary Tract Infection, and their Antibiotic Resistance Pattern

Authors

  • Abdolahzadeh, Zahra Department of Microbiology, School of Medicine, Shahid Sadoghi University of Medical Sciences and Health Services, Yazd, Iran.
  • Astani, Akram Department of Microbiology, School of Medicine, Shahid Sadoghi University of Medical Sciences and Health Services, Yazd, Iran.
  • Mosadegh, Ahmad Department of Microbiology, School of Medicine, Shahid Sadoghi University of Medical Sciences and Health Services, Yazd, Iran.
  • Vakili, Mahmoud Department of Microbiology, School of Medicine, Shahid Sadoghi University of Medical Sciences and Health Services, Yazd, Iran.
Abstract:

Introduction: E. coli is the predominant causes of urinary tract infection. Several virulence factors for bacterial infections in the urinary tract are required. In this study, we have considered several virulence factors in strains isolated from the patients with UTI in Yazd. Methods: In this cross-sectional study in 1394-1395, 146 strains isolated were collected from the patients with urinary tract infection. After confirmation by phenotypic and genotype methods, frequency of gene fimH, pap C, aer was evaluated using specific primers by PCR method. The pattern of antibiotic resistance of isolates was determined by disk diffusion method. Results: In the present study, the prevalence of virulence genes was fimH (87%), aer (85.6%) and papC (9.6%). Among 146 E. coli isolates, resistance rate for various antibiotics were as follows: 57.2% to Cotrimoxazole, 54.8% to Nalidixic acid, 45.9% to Cefazolin, 40.4% to Cefixime, 42.5% to Cefalotin, 41.8% to Cefalexin, 31.5% to Norfloxacin, 30.1% to Ofloxacin, 28.3% to Ciprofloxacin, 24% to Gentamicin, 19.9% to Amikacin, 1.4% to Nitrofurantoin, and 1.4% to  phosphomycin. The results showed that the most and less frequent resistant was seen in Cotrimoxazole and Nitrofurantoin, phosphomycin, respectively. Conclusion: In this study, the frequency of fimH gene was higher than other genes. In addition, according to the pattern of antibiotic resistance nitrofurantoin and phosphomycin are suitable medicines for the treatment of patients in our region.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Antibiotic resistance pattern and serotyping of Escherichia coli producing siderophore in people with urinary tract infection

Urinary tract infection is one of the most common bacterial infections of human. The most common agent of urinary tract infection is Escherichia coli . This study aims to determine the prevalence of uropathogenic E. coli urinary infection in human with different antimicrobial resistance, and quantitive and qualitative study of siderophore production and their association with the ability to cau...

full text

Investigation of fimH Single Nucleotide Polymorphisms (C640T and T591A) in Uropathogenic E. coli Isolated from Patients with Urinary Tract Infections

Background: Urinary tract infections are one of the most frequent health problems and Uropathogenic Escherichia coli is the major pathogen resulting UTIs. The severity of UTIs is caused by the expression of a large range of virulence factors.In this study, we evaluated the allelic frequency fimH gene, in UPECs isolated from patients with UTIs. This study also aimed to determine the roles of C64...

full text

Antibiotic Resistance Pattern of Different Escherichia coli Phylogenetic Groups Isolated from Human Urinary Tract Infection and Avian Colibacillosis

Background: The emergence and propagation of different phylogenetic groups of antimicrobial-resistant E. coli have become a worldwide health concern in human and veterinary medicine. Therefore, the evaluation of the phylogenetic distribution of antibiotic-resistant E. coli is important for therapeutic and economic purposes. The aims of this study were to determine phylogenetic groups and patter...

full text

The Detection of Fimbrial Pathogenic Genes in E. coli Strains Isolated from Patients with Urinary Tract Infection

Abstract Bachground and objectives: The ability of adherence to the surface of host cell is very critical in the colonization of microbial pathogens. It has been revealed that E. coli strains that infect urinary tracts have different fimbrea such as I, S, FIC, Dr, and fimbrial adhesions. Material and Methods: In this study, 363 urine samples were obtained from patients with urinary tract infect...

full text

the fimh gene in uropathogenic escherichia coli strains isolated from patients with urinary tract infection

conclusions the results of this study indicated that more than 90% of e. coli isolates harbored the fimh gene. the high binding ability of fimh could result in the increased pathogenicity of e. coli; thus, fimh could be used as a possible diagnostic marker and/or vaccine candidate. materials and methods a total of 140 isolated e. coli strains from patients with uti were identified using biochem...

full text

The FimH Gene in Uropathogenic Escherichia coli Strains Isolated From Patients With Urinary Tract Infection

BACKGROUND Urinary tract infections (UTIs) are one of main health problems caused by many microorganisms, including uropathogenic Escherichia coli (UPEC). UPEC strains are the most frequent pathogens responsible for 85% and 50% of community and hospital acquired UTIs, respectively. UPEC strains have special virulence factors, including type 1 fimbriae, which can result in worsening of UTIs. O...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 29  issue 3

pages  3556- 3565

publication date 2021-05

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

No Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023