Food Safety Evaluation of Imidacloprid Residues in Grape Berries at a Different Dose of Spraying

Background & Aims of the Study: Grape, a crucial agriculture crop of Malayer city, is affected by Vine cicada, Psalmocharias alhageos.  Imidacloprid, a neonicotinoid insecticide, provides a good management of this insect. The aim of this research is residue persistence study of imidacloprid on grapes, to estimate its residue deposit, the half-life of degradation and safe pre-harvest consumption time. Materials & Methods: Residues of imidacloprid were estimated in grape following two recommended types of spraying (80.0 g a.i. ha-1) and duplicates the application rate (160.0 g a.i. ha-1). Samples were collected at 1 h to 21 days after spraying of imidacloprid. The analyses were done by the QuECHERS technique, using HPLC-UV. Results: The average initial concentration of imidacloprid on grapes found to be 10.58 and 17.56 mg kg-1 at single and double dosages, respectively. These residues of imidacloprid decreased to 97.8% and 98.0%, respectively, at single and double dosages in 15 days, with a half-life period of 2.21 and 2.94 days. Residues of imidacloprid on grapes were less than its MRL value after 7 and 10 days of recommended and double dosage of spraying. Residues of imidacloprid in grape berries at harvest were discovered to below the determination limit. Conclusions: Consequently, a waiting time of 7 and 10 days is usually recommended for safe consumption of grapes once imidacloprid spraying. Acceptable daily intake (ADI) of imidacloprid is 0.06 mg kg-1 body weight day-1. According to the results of this study, the employment of imidacloprid on the grape looks to be toxicologically acceptable.