Fermentative Production of Lysine by Corynebacterium glutamicum from Different Carbon Sources

Authors

  • M. MOOSAVI-NASAB Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, I.R. Iran
  • S. ANSARI Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, I.R. Iran
  • Z. MONTAZER Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, I.R. Iran
Abstract:

Production of lysine by Corynebacterium glutamicum (PTCC 1532) from different agricultural by-products (molasses and pulpy waste date) was compared to glucose as raw materials. For this purpose, ammonium sulphate was selected as a constant nitrogen source. The effect of different nitrogen sources was also investigated with glucose as a constant carbon source. The production of L-lysine was examined qualitatively and quantitatively using thin layer chromatography (TLC). Results of fermentation experiments showed that the maximum yield corresponded to molasses (48 g L-1) for the fermentation period of 96 hours. For other substrates the yield was lower and the period of fermentation exceeded that for molasses.

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fermentative production of lysine by corynebacterium glutamicum from different carbon sources

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Journal title

volume 25.26  issue 1.2

pages  99- 106

publication date 2008-03-19

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