Expression of Drug Pump Protein MRP2 in Lipopolysaccharide-Treated Rats and Its Impact on the Disposition of Acetaminophen

Authors

  • Jamaal Kasaeeian Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Javaad Behravan Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Mitra Hassany Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Mohammad Hassanzadeh Khayat Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Pharmaceutiacal Sciences research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract:

The drug pump protein MRP2 is a membrane drug efflux transporter widely distributed in normal and tumor tissues. Its role is thought to be crucial for the disposition of many drugs and their substrates in different tissues. In this study, we aimed to examine the effects of systematic inflammation induced by lipopolysaccharide (LPS) on the expression and function of this transporter in rats. Jugular cannulated rats were injected intraperitoneally with LPS. Control rats received equal volume of sterile saline buffer. Rat liver MRP2 expression was analyzed at the level of mRNA through reverse transcription polymerase chain reaction. At various time points following drug administration (15 to 360 min), 250 µL blood samples were obtained from the cannula. The plasma was separated by centrifugation and stored at -20°C until HPLC analysis. Administration of LPS resulted in a slight but not significant increase in MRP2 mRNA levels 24 h after the treatment. In HPLC analysis, a rapid decrease in plasma concentrations of the MRP2 substrate (APAP) was observed at the initial time points. At later time points, the slope of the substrate concentration reached a plateau and paralleled to those of controls. It was postulated that due to the presence of the possible compensatory transport mechanisms in liver and non-hepatic tissues, changes performed to the pump activity were not completely in parallel to the expression of the drug efflux pump.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

expression of drug pump protein mrp2 in lipopolysaccharide-treated rats and its impact on the disposition of acetaminophen

the drug pump protein mrp2 is a membrane drug efflux transporter widely distributed in normal and tumor tissues. its role is thought to be crucial for the disposition of many drugs and their substrates in different tissues. in this study, we aimed to examine the effects of systematic inflammation induced by lipopolysaccharide (lps) on the expression and function of this transporter in rats. jug...

full text

Expression of Drug Pump Protein MRP2 in Lipopolysaccharide-Treated Rats and its Impact on the Disposition of Acetaminophen

The drug pump protein MRP2 is a membrane drug efflux transporter widely distributed in normal and tumor tissues. Its role is thought to be crucial for the disposition of many drugs and their substrates in different tissues. In this study, we aimed to examine the effects of systematic inflammation induced by lipopolysaccharide (LPS) on the expression and function of this transporter in rats. Jug...

full text

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

an investigation of the impact of self monitoring on langauge teachers motivational practice and its effect on learners motivation

the central purpose of this study was to conduct a case study about the role of self monitoring in teacher’s use of motivational strategies. furthermore it focused on how these strategies affected students’ motivational behavior. although many studies have been done to investigate teachers’ motivational strategies use (cheng & d?rnyei, 2007; d?rnyei & csizer, 1998; green, 2001, guilloteaux & d?...

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume Volume 10  issue 4

pages  855- 859

publication date 2011-09-13

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023