Evaluation the effects of Teucrium polium hydroalcoholic extract on the fetus and chorioallantoic fluid using a chick embryo model

Introduction Side effects of drugs have always been controversial issues in medical science. To date, sufficient researches were not performed on the side and pathological effects of Teucrium polium as an herb in the fetus. Therefore, the present study aimed to investigate the pathological effects of Teucrium polium hydroalcoholic extract on chorioallantoic fluid composition, weight, growth, external and internal tissues of the fetus using a chick embryo model. Materials and Methods A total of 48 Ross 308 embryonated eggs were randomly divided into 3 equal groups. In the first group (control), 0.5 CC of phosphate buffer saline and the second and third groups, Teucrium polium hydroalcoholic extract with doses of 25 and 50 mg/kg egg-weight were inoculated into the eggs. Embryos were allowed to develop until day 18 and then examined for macroscopic and microscopic lesions. Results Injection of e hydroalcoholic extract caused a disturbance in the weight, growth, and biochemical factors of the chorioallantoic fluid during the growth period. The most pathological effects were observed in the brain, lung, heart, liver, and kidney of the embryos which received the Teucrium polium hydroalcoholic extract at a dose of 50 mg/kg. Conclusion Teucrium polium hydroalcoholic extract can produce various pathological effects in the external organ, internal organ, and the chorioallantoic fluid. Considering the consumption of this herb in different regions of Iran, it is recommended that its usage be taken with caution, especially during pregnancy.