Evaluation of Stability of Chitinase Gene in Transgenic Offspring of Cotton (Gossypium hirsutum)

Authors

  • Behzad Ghareyazie Agricultural Biotechnology Research Institute of Iran (ABRII) P.O. Box 31535-1897, karaj, I.R. Iran
  • Hassan Rassouli Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, P.O. Box 313, Zanjan, I.R. Iran
  • Javad Najafi Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, P.O. Box 313, Zanjan, I.R. Iran
  • Masoud Tohidfa Agricultural Biotechnology Research Institute of Iran (ABRII) P.O. Box 31535-1897, karaj, I.R. Iran
Abstract:

Cotton cultivar Coker has been already transformed with recombinant pBI121-chi via Agrobacterium tumefaciens. The T-DNA region of pBI121-chi carries the chitinase (chi ) gene from bean and is under the control of the CaMV35S promoter. T1 and T2 progenies of transgenic cotton containing the chi gene were used in this study.  Polymerase chain reaction (PCR), Southern and Western blotting data confirmed integration and expression of the chi gene in the T1 and T2 progenies. The growth of Verticillium dahliae was singnificantly inhibited in an in vitro bioassay for which 100 μg of crude leaf protein extract derived from the T1 plants was used. The 850-bp expected chi fragment was amplified for 77 transgenic plants from 128 T1 and T2 progenies, and 75 transgenic plants showed both chi and nptII bands. T0 conduct bioassay, cotton seedlings were infected with the spore suspension (106 spores/ml), in a greenhouse. Fifty-five percent of the transgenic plants were able to restrict V. dahliae growth and symptoms. There were no distinguishable differences in the phenotypic appearance of transgenic plants compared to non-transgenics. These results showed that transgenic cotton expressing a bean chitinase exhibited enhanced resistance against  V. dahliae in greenhouse and in-vitro assay as compared to the non-transgenic plants.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

evaluation of stability of chitinase gene in transgenic offspring of cotton (gossypium hirsutum)

cotton cultivar coker has been already transformed with recombinant pbi121-chi via agrobacterium tumefaciens. the t-dna region of pbi121-chi carries the chitinase (chi ) gene from bean and is under the control of the camv35s promoter. t1 and t2 progenies of transgenic cotton containing the chi gene were used in this study.  polymerase chain reaction (pcr), southern and western blotting data con...

full text

evaluation of sadr eminence in safavid period

چکیده: یکی از دوره های مهم تاریخی ایران به لحاظ تأمین استقلال ملی مذهبی و حتی تأثیر آن بر فرهنگ و مذهب ایرانیان، دوره صفویه است. رسمیت دادن و رواج مذهب شیعه توسط شاه اسماعیل اول، یکی از مهمترین اقدامات این دولت محسوب می شود. بنابراین برای اجرای این سیاست، وی منصب صدارت را به عنوان منصبی مذهبی- حکو متی ایجاد کرد .این منصب از دوره ی تیموریان ایجاد شده بود ولی در اواخر این دوره اهمیت بیشتری یافت...

15 صفحه اول

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 7  issue 1

pages  45- 50

publication date 2009-01-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023