Evaluation of Kohlrabi (Brassica oleracea var. Gongylodes) extract effect on mesenchymal stem cell viability and apoptosis

Authors

  • Ali Kowsari Department of Mesenchymal Stem Cell, The Academic Centre for Education, Culture and Research, Qom, Iran.
  • Mohsen Sheykhhasan Department of Mesenchymal Stem Cell, The Academic Centre for Education, Culture and Research, Qom, Iran.
  • Naser Kalhor Qom Department of Mesenchymal Stem Cell, The Academic Centre for Education, Culture and Research, Qom, Iran.
Abstract:

Background: Cell viability and apoptosis are two crucial factors that may determine cell fate. There are several factors, such as hypoxia, which may be effective in cell processes. Because of its unique features, such as its antioxidant, anti-inflammatory, and anti-apoptosis mechanisms, kohlrabi (Brassica oleracea var. gongylodes) extract may be used in the amelioration of cell viability and a decrease in cell apoptosis. In this study, we evaluate the effect of kohlrabi extract on the viability and apoptosis of adipose-derived mesenchymal stem cells (AD-MSCs). Material and Methods: In this study, extract from kohlrabi and mesenchymal stem cells from adipose tissue were isolated in a laboratory under sterile conditions. Expression of mesenchymal stem cell (MSC) surface markers, including CD44, CD90, and CD105 was evaluated by flow cytometry method. Besides, CD34 was used as a negative marker. MTT assay was carried out to determine the cell viability. Evaluation of BCL2 and BAX expression levels was performed by real-time PCR. Results: MSC surface markers were verified by flow cytometry. The obtained results demonstrated a significant difference between the cell viability of the kohlrabi-extract treated and control group over time (P=0.03). In addition, the real-time PCR analysis showed that expression levels of BCL2 significantly increased in hypoxic condition after treatment with leaf extract (P=0.019). However, there was no significant expression change in the BAX gene. Conclusion: Our study illustrates that kohlrabi extract may have positive effects on cell survival while having inhibitory effects on apoptosis.  

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Quality of kohlrabi stems (Brassica oleracea var. gongylodes L.) kept in cold storage

Two green kohlrabi cultivars, ‘White Delikates’ and ‘Korist’ F1, were kept in cold storage at a temperature of 2oC and a relative humidity of 95%. Natural mass losses were measured at monthly intervals and dry matter content, soluble sugars, L-ascorbic acid and isothiocyanates were analysed. During five months of storage, very low losses of kohlrabi mass were detected. The decrease in dry matte...

full text

Anti-Diabetic and Anti-Inflammatory Effects of Green and Red Kohlrabi Cultivars (Brassica oleracea var. gongylodes)

The aim of the present study was to evaluate the anti-diabetic, anti-inflammatory, antioxidant potential, and total phenolic content (TPC) of green and red kohlrabi cultivars. Anti-diabetic and anti-inflammatory activities were evaluated via protein tyrosine phosphatase (PTP1B) and rat lens aldose reductase inhibitory assays and cell-based lipopolysaccharide (LPS)-induced nitric oxide (NO) inhi...

full text

Application of crude extract of kohlrabi (Brassica oleracea gongylodes) as a rich source of peroxidase in the spectrofluorometric determination of hydrogen peroxide in honey samples.

Crude extract of kohlrabi (Brassica oleracea gongylodes) was prepared by a simple procedure and its enzymatic activity and total protein concentration were determined. It was found that this crude extract is a rich source of peroxidase (POx) and has high specific activity. Cross-linked polyvinylpyrrolidone was used as a stabilizer in the preparation of the crude extract. The POx activity of koh...

full text

The Use of Crude Extract of Kohlrabi (Brassica oleracea gongylodes) as a Source of Peroxidase in the Spectrofluorimetric Determination of Thiamine

The crude extract of kohlrabi (Brassica oleracea gongylodes) was prepared as a rich source of peroxidase and its enzymatic and specific activities in the presence of cross-linked polyvinylpyrrolidone as a stabilizer were determined. This extract was used to catalyze the oxidation of thiamine in the presence of hydrogen peroxide to thiochrome at pH 8.0. Thiochrome shows strong fluorescence at 43...

full text

Antihyperglycemic, antihyperlipidemic and antioxidant activity of phenolic rich extract of Brassica oleraceae var gongylodes on streptozotocin induced Wistar rats

Cruciferous vegetables, in particular those included into the Brassica genus, are good sources of a variety of nutrients and health-promoting phytochemicals. Phenolic compounds are the major antioxidants of Brassica; hence the contribution of Brassica vegetables to health improvement has largely been associated to their antioxidant capacity. This study aimed to assess anti-diabetic, antilipidem...

full text

Chromosome Doubling of Microspore-Derived Plants from Cabbage (Brassica oleracea var. capitata L.) and Broccoli (Brassica oleracea var. italica L.)

Chromosome doubling of microspore-derived plants is an important factor in the practical application of microspore culture technology because breeding programs require a large number of genetically stable, homozygous doubled haploid plants with a high level of fertility. In the present paper, 29 populations of microspore-derived plantlets from cabbage (Brassica oleracea var. capitata) and brocc...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 8  issue 2

pages  0- 0

publication date 2020-05

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023