Electrophoretic Pattern and Antibacterial Activity of Proteins from Vicia Faba Seed Extract

ABSTRACT Background Antibiotic resistance makes antimicrobial peptides (AMPs) agents an alternative for treatment of pathogenic diseases. They are isolated from various animals invertebrates, vertebrates and plants. The present study shows the electrophoretic pattern of protein and peptides from Vicia Faba seed and reports our first attempt to study the antibacterial activity of Vicia faba seed extract. Methods 100g grounded seed was extracted in extraction buffer(10 mM Na2HPO4, 15 mM NaH2PO4, 100mM KCl, 2 mM EDTA, 2mM Thiurea 1.5% PVPP). Following a 70% ammonium sulfate precipitation was carried out. The pellet was dialyses and resuspended in 50mM ammonium acetate, then used for gel filtration chromatography (Sephadex G75). The crude extract electrophoresis was carried out on 12% SDS- PAGE gel. Antibacterial activity on E.Coli and B. Subtilis from hospital infection was tested and evaluated by measuring the inhibition zone diameter observation. Results The SDS- PAGE gel electrophoresis shows the crude extract contains many proteins with different spectrum molecular weight of proteins and peptides. The inhibition zone doesn’t observe in antibacterial properties tests. So, our experiments don’t show any antibacterial activity on E.Coli and B. Subtilis from hospital infection. Conclusion In our study, bacteria from hospital infection resists to Vicia faba seed extract. Some other AMPs haven’t also shown any antimicrobial properties on clinical trail. To show the antibacterial activity of the crude Vicia faba seed extract, it should be tested on some standard bacteria.