Effects of Human Adipose-derived Stem Cells and Platelet-rich Plasma on Healing Response of Canine Alveolar Surgical Bone Defects

Authors

  • Alireza Ebrahimzadeh-Bideskan Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Microanatomy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Alireza Fazel Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Microanatomy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Amin Rahpeyma Oral and Maxillofacial Diseases Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran
  • Ariane Sadr-Nabavi Department of Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Halimeh Hassanzadeh Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran Stem Cells and Regenerative Medicine Research Group, Academic Center for Education, Culture, and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran
  • Hamideh Salari Sedigh Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
  • Maryam Moghadam Matin Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran Stem Cells and Regenerative Medicine Research Group, Academic Center for Education, Culture, and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
  • Reyhaneh Shafieian Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract:

Background: Due to the known disadvantages of autologous bone grafting, tissue engineering approaches have become an attractive method for ridge augmentation in dentistry. To the best of our knowledge, this is the first study conducted to evaluate the potential therapeutic capacity of PRP-assisted hADSCs seeded on HA/TCP granules on regenerative healing response of canine alveolar surgical bone defects. This could offer a great advantage to alternative approaches of bone tissue healing-induced therapies at clinically chair-side procedures. Methods: Cylindrical through-and-through defects were drilled in the mandibular plate of 5 mongrel dogs and filled randomly as following: I- autologous crushed mandibular bone, II- no filling material, III- HA/TCP granules in combination with PRP, and IV- PRP-enriched hADSCs seeded on HA/TCP granules. After the completion of an 8-week period of healing, radiographic, histological and histomorphometrical analysis of osteocyte number, newly-formed vessels and marrow spaces were used for evaluation and comparison of the mentioned groups. Furthermore, the buccal side of mandibular alveolar bone of every individual animal was drilled as normal control samples (n=5). Results: Our results revealed that hADSCs subcultured on HA/TCP granules in combination with PRP significantly promoted bone tissue regeneration as compared with those defects treated only with PRP and HA/TCP granules (P

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

The Effect of Human Platelet-Rich Plasma on Adipose-Derived Stem Cell Proliferation and Osteogenic Differentiation

Background: The cultured mesenchymal stem cells (MSC) have been used in many clinical trials however, there are still some concerns about the cultural conditions. One concern is related to the use of FBS as a widely used xenogeneic supplement in the culture system. Human platelet-rich plasma (hPRP) is a candidate replacement for FBS. In this study, the effect of hPRP on MSC proliferation and os...

full text

The effect of platelet-rich plasma on human mesenchymal stem cell-induced bone regeneration of canine alveolar defects with calcium phosphate-based scaffolds

Objective(s): Autologous bone transplantation known as the “gold standard” to reconstruction of osseous defects has known disadvantages. This study was designed to explore the effects of hydroxy-apatite/tricalcium-phosphate (HA/TCP) and platelet-rich plasma (PRP) on the osteogenesis ability of human adipose-derived mesenchymal stem cells (hAdMSCs) in vitro and in vivo. Materials and Methods: hA...

full text

The Effect of Platelet Rich Plasma on Chondrogenic Differentiation of Human Adipose Derived Stem Cells in Transwell Culture

  Objective(s): Platelet-rich plasma (PRP) has recently emerged as a promising strategy in regenerative medicine due to its multiple endogenous growth factors. Little is known about the role of PRP as a promoter in chondrogenesis of human adipose derived stem cells (hADSCs). The aim of this study was to determine whether PRP may be considered as a natural and easy achievable source o...

full text

the effect of human platelet-rich plasma on adipose-derived stem cell proliferation and osteogenic differentiation

background: the cultured mesenchymal stem cells (msc) have been used in many clinical trials however, there are still some concerns about the cultural conditions. one concern is related to the use of fbs as a widely used xenogeneic supplement in the culture system. human platelet-rich plasma (hprp) is a candidate replacement for fbs. in this study, the effect of hprp on msc proliferation and os...

full text

the effect of platelet rich plasma on chondrogenic differentiation of human adipose derived stem cells in transwell culture

objective(s): platelet-rich plasma (prp) has recently emerged as a promising strategy in regenerative medicine due to its multiple endogenous growth factors. little is known about the role of prp as a promoter in chondrogenesis of human adipose derived stem cells (hadscs). the aim of this study was to determine whether prp may be considered as a natural and easy achievable source of growth fact...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 5  issue 6

pages  406- 418

publication date 2017-11-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023