Effects of assisted oocyte activation with calcium- ionophore and strontium chloride on in vitro ICSI outcomes

Authors

  • Leila Azadi Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
  • Marziyeh Norozi-Hafshejani Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
  • Marziyeh Tavalaee Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
  • Mohamad Hosein Nasr Esfahani Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran|Isfahan Fertility and Infertility Center, Isfahan, Iran
Abstract:

Objective(s): Failed fertilization after intra-cytoplasmic sperm injection (ICSI) is mainly attributed to failed oocyte activation and can be overcome by artificial oocyte activation (AOA). The present study aims to compare in vitro outcomes of ICSI following two different assisted oocyte activation chemical procedures (SrCl2 and Ionomycin) in sibling oocytes of ICSI candidates.Materials and Methods: From March 2015 until February 2016, 105 infertile men with 99–100% abnormal sperm morphology, irrespective of sperm motility, concentration, or origin (semen or testicular) were included in this study. Out of these, 66 couples accepted to be included in the study group (Ionomycin/ SrCl2) and 39 couples requested routine AOA procedure (Ionomycin) as external control group. Primary outcomes of this study (fertilization, embryo quality, and post-implantation development) were compared between these groups.Results: Significantly higher oocyte activation (67.90±3.6% vs. 51.16±3.6%, P=0.004) and fertilization (65.23±3.63% vs. 49.65±3.63%, P=0.008) rates were observed in sibling oocytes treated with Ionomycin in comparison to the SrCl2 sibling group. Percentage of top quality embryos was insignificantly higher in SrCl2 groups compared to the Ionomycin group (29.90±4.27 vs. 20.65±4.05%, P=0.26).Conclusion: Ionomycin may be superior to SrCl2 for inducing oocyte activation. However, SrCl2 may be a more efficient means to support the development of better quality embryos following ICSI.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

effect of oocyte activation with calcium ionophore on icsi outcomes in teratospermia: randomized clinical trial

oocyte activation (aoa), results in high fertilization rates. objective: this prospective, randomized, unblinded clinical study aimed to evaluate the efficiency of calcium ionophore on fertilization and pregnancy rate after intracytoplasmic sperm injection (icsi) in infertile men suffer from teratoospermia. materials and methods: thirty eight women with teratoospermic partner underwent icsi wit...

full text

Effect of oocyte activation with calcium ionophore on ICSI outcomes in teratospermia: A randomized clinical trial

BACKGROUND Chemical activation is the most frequently used method for artificial oocyte activation (AOA), results in high fertilization rate. OBJECTIVE This prospective, randomized, unblinded, clinical study aimed to evaluate the efficiency of oocyte activation with calcium ionophore on fertilization and pregnancy rate after intracytoplasmic sperm injection (ICSI) in infertile men suffer from...

full text

P-97: Parthenogenetic Activation of Mouse Oocyte Using Calcium Ionophore in The Presence of Different Concentrations of Extracellular Calcium

Background: Parthenogenetic activation is a possible way to produce homogeneous embryos with the some ploidy. Probably such embryos could be used in other areas of biotechnology. Calcium signals are known as important regulators of oocyte activation. Extracellular calcium is required for initiation of meiotic resumption and development. Calcium ionophore A23187 is known to elevate intracellular...

full text

P-24: The Effect of Oocyte Activation with DTT on Development of Ovine Embryos in ICSI

Background: The objective of the current study was to determine the effect of activation of injected oocytes with dithiothreitol on development of ovine embryos derived from in vitro-matured oocytes and intracytoplasmic sperm.The injected oocytes with non-treated sperm were activated with 1) ionomycin (Io) + 6-dimethylaminopurine (6-DMAP) 2) DTT, 3) DTT+ ionomycin (I)+6-DMAP. Materials and Meth...

full text

a successful healthy childbirth in a case of total globozoospermia with oocyte activation by calcium ionophore

background: globozoospermia is a rare (incidence of 0.1% among andrological patients) and poorly understood condition, but a severe disorder in male infertility. this case report detailed the course of treatment and protocol of a patient with type 1 globozoospermia using intracytoplasmic sperm injection (icsi) and oocyte activation by calcium ionophore, which yielded conception and birth of a h...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 21  issue 11

pages  1109- 1117

publication date 2018-11-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023