Effectiveness of Antibacterial Properties of Mentha Pulegium Hydroalcoholic Extract on Pathogens by Microdilution in Vitro

Background: The use of antimicrobial properties of medicinal plants can solve the problems associated with the use of antibiotics. The aim of this study was to evaluate the antibacterial property of Oregano hydro alcoholic extract on pathogen bacteria by microplate in laboratory conditions. Purpose: The aim of this study was to evaluate the antibacterial properties of hydro alcoholic extract of oregano on pathogenic bacteria by microplate method in vitro.  Methods: After collecting oregano, they were dried in a place by sunlight and in shade for 48-48 hours, and extraction was carried out by maceration, in order to determine the amount of inhibition (dilution) inhibitor The Muller-Hinton broth was used. The turbidity rate was evaluated in the wells method (microdilution). Conclusion: The results of microdilution test showed that the minimum inhibitory concentration of extract on Escherichia coli in the first (lµ50), the concentration of extract on salmonella typhimurium in the first (lµ50), the concentration of Klebsiella pneumonia in the second (lµ25) , Concentrations of Pseudomonas aeruginosa in the sixth poison (lµ1.56), concentration of Bacillus cereus in the eighth grade (lµ0.39), and the minimum concentration of Staphylococcus aureus in the tenth (without growth) strain. The results showed that Oregano essential oil has antibacterial properties and can be used as a cheap and available source for therapeutic applications in some bacterial infections. In other words, Oregano can be considered as an alternative to synthetic antibiotics to combat the isolates tested. Of course, all the effects of these extracts should be carefully investigated in vivo and in vitro.