Effect of Bacillus subtilis IS02 in food on health, immunity indexes and prevention against White Spot Disease in Litopenaeus vannamei

    In this study, “Tak Cell” probiotic, based on eight-year studies (2009- 2016) of the Iranian Fisheries Sciences Research Institute in National Shrimp Research Institute, and produced in collaboration with the “Tak Zhen Zist ®” company, was added to the diet of western white shrimp (Litopenaeus vannamei) weighing more than 6 grams (in three Different quantities 106, 107 and 108 CFU/Kg) and health indicators (including THC, TPP and DHC) were determined at the beginning and the end of the test period. For this purpose, shrimp was randomly selected and kept in 150-liter aquariums (30 Shrimps in each aquarium). The Shrimps were treated in three treatments and each with three replications and negative control (no probiotic and disease agent) and positive control (With probiotic). In each treatment, shrimps were fed with the “Tak Cell” probiotic pellet three times a day for 40 days and the parameters of temperature; salinity and oxygen were recorded during the test. After 40 days, exposure to white spot virus was challenged and daily mortality and survival rate was recorded for 2 weeks. According to Robertson et. al key, shrimp in the phase between two molting selected and hemolymph sampling was Carried out and the health index, including Total Hemocyte Count (THC), Differentiated Hemocyte Count (DHC) and Total Plasma Protein (TPP) were measured and compared with positive and negative control groups. According to the results, concluded that feeding Litopenaeus vannamei with diets containing 107 and 108 CFU/Kg were significantly increase (P≤0.05). The performance of shrimp immune system and health indicators. In addition, the fed of these diets is effective in increasing the survival rate of shrimp exposure to white spot virus in comparison with the control (P<0.05).