Effect of Administration Exogenous Leptin on Reactive Oxygen Species Level in Seminal Plasma Male Sprague-Dawley Rats

Purpose: The Increase of oxidative stress production a causes after oxygen free radicals one of the factors involved in male infertility. In this study the role of leptin in product reactive oxygen species in seminal plasma in adult rat were evaluated. Materials and Methods: Sixty-five male rats were randomly divided into four groups. The control group receives normal saline and other animal, in experimental groups 2, 3 and 4 were given daily intraperitoneal leptin injections of 5, 10, or 30 mg/kg body weight for 7, 15 and 42 at all. Reactive Oxygen Species Levels and Expression of leptin were measured respectively with DCFH-A and immunohistochemical and sperm parameters were assessed by computer-assisted semen analysis (CASA). Two ways ANOVA with Tukey post hoc test were conducted to compare mean of groups. P-value<0.05 was considered significant. Result: The results show that sperm count was higher in treated groups than that of control group. These differences were significant (p£0.05). The interaction between treatment and time showed significant effect on ROS variations.After immune staining leptin were found in the seminiferous, tubules and Leptin receptor were found in the interstitium.Conclusion: the results of this study indicate that leptinadministration by increasing levels of oxygen free radicals, may lead toincrease of sperm count and the number of abnormal sperm in the experimental groups.