Detection of specific antigens of Newcastle disease virus using an absorbed Western blotting method

Detection of specific antigens of Newcastle disease virus using an absorbed Western blotting method.

Newcastle disease virus (NDV) is an economically important poultry pathogen with a worldwide distribution that may infect a wide range of domestic and wild avian species. The identification of different pathotypes of NDVs plays an important role in the diagnosis and development of vaccines to control and eradicate NDV infections. In our previous study, we showed that mono-specific antibodies ca...

western blotting method for diagnosis of hydatid cyst antigens

determination of diagnostic antigens in cattle amphistomiasis using western blotting

background: mixed infection with amphistomes seems common in native cattle of iran. the aim of this study was to determine diagnostic antigens in cattle mixed amphistomiasis. methods: specific antigens of cotylophoron cotylophorum , gastrothylax crumenifer and paramphisto­mum cervi (mixed infection), the most common species, were collected from cattle was deter­mined. adult trematodes were coll...

Immunofluorescent protein detection in Western blotting

This report describes the detailed procedures for Western blot analysis using fluorescent antibodies. After electrophoresis and subsequent electroblotting, the fluorescent-labeled antibodies were visible upon ultraviolet illumination of the polyvinylidene fluoride (PVDF) membranes and could then be photographed to give an accurate record of the blots. Fluorescent labeling allows for photographi...

buckling of viscoelastic composite plates using the finite strip method

در سال های اخیر، تقاضای استفاده از تئوری خطی ویسکوالاستیسیته بیشتر شده است. با افزایش استفاده از کامپوزیت های پیشرفته در صنایع هوایی و همچنین استفاده روزافزون از مواد پلیمری، اهمیت روش های دقیق طراحی و تحلیل چنین ساختارهایی بیشتر شده است. این مواد جدید از خودشان رفتارهای مکانیکی ارائه می دهند که با تئوری های الاستیسیته و ویسکوزیته، نمی توان آن ها را توصیف کرد. این مواد، خواص ویسکوالاستیک دارند....

Detection of midgut antigens of Hyalomma anatolicum anatolicum tick using SDS-PAGE and Western blot

Ticks are important ectoparasites which are a considerable threat to human beings and to animals all overthe world. Enormous economic losses annually occur in livestock production around the world as a result oftheir existance. One of the ways to control ticks and tick-borne diseases is to introduce resistance to theseectoparasites through immunization. For identification of the putative protec...