Detection of midgut antigens of Hyalomma anatolicum anatolicum tick using SDS-PAGE and Western blot

detection of midgut antigens of hyalomma anatolicum anatolicum tick using sds-page and western blot

ticks are important ectoparasites which are a considerable threat to human beings and to animals all overthe world. enormous economic losses annually occur in livestock production around the world as a result oftheir existance. one of the ways to control ticks and tick-borne diseases is to introduce resistance to theseectoparasites through immunization. for identification of the putative protec...

Immunization of cattle against Hyalomma anatolicum anatolicum using larval antigens.

Development of immunity in cross-bred (Bos taurus x Bos indicus) calves against Hyalomma anatolicum anatolicum, vector of bovine tropical theileriosis, was studied using larval antigen (LS) in Freund's complete adjuvant (FCA). Calves immunized with LS + FCA showed significant rejection of larvae (57.25 +/- 6.8) and nymphs (45.75 +/- 5.16). Abnormally fed larvae (11.4 +/- 0.8) and nymphs (8.25 +...

Preparation of antigens from midgut of Hyalomma anatolicum anatolicum and determining their immunoprotective effects

  Partially fed (4 -5 days) H .a. anatolicum (Iranian isolate) female adult ticks were used for preparation of antigens of midgut which are, the gut supernate antigen (GS), gut membrane antigen (GM), triton extracted gut membrane antigen ( TX-GM ) and sodium dodecyl sulphate treated Triton extracted gut membrane antigen (SDS-TX-GM). The New Zealand white rabbits were immunized with GS, GM, TX- ...

Proteases Detection of invitro Culture of Midgut Cells from Hyalomma anatolicum anatolicum (Acari: Ixodidae)

  Proteases play a key role in protein digestion in ticks and other haematophagous insects. Our understanding of blood meal digestion in digestive system of ticks can be very useful for better understanding of basic rules for control of ticks. Cells of the midgut endocytose blood components. Blood proteins uptake by midgut cells, suggesting the presence of proteases in the midgut cells. In this...

High infection rates of the tick Hyalomma anatolicum anatolicum with Trypanosoma theileri.

A crossbred calf (3 months old) obtained from a farm where regular control of ticks was practised and found to be free of blood parasites was inoculated with 20 ml pooled blood collected from four field cattle which had very low Trypanosoma theileri parasitaemias (one parasite per 70 microl blood as determined by the haematocrit centrifugation technique). Trypanosoma theileri was present in the...

Effect of Tick Gut Extract on Protection of Cattle against Hyalomma anatolicum anatolicum