Detection of Methicillin Resistant Staphylococcus aureus (MRSA) by CHROMagar Versus Cefoxitin Disk Diffusion Method

author

  • Hossein Koupahi Department of Microbiology, Islamic Azad University, Varamin- Pishva branch, Iran.
Abstract:

 Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene to detection of MRSA. A total of 220 clinical isolates of S. aureus which were isolated from various clinical specimens from 1 August 2018 to 30 June 2019 in Milad Hospital of Tehran, Iran was subject of this study. Methicillin resistance was determined by cefoxitin disk, CHROMagar™ MRSA medium and detection of the mecA gene by Polymerase Chain Reaction (PCR) as the gold standard method. Among 220 isolates from S. aureus, 105 (47.72%) isolates were positive for mecA gene by PCR method. The results of cefoxitin disk diffusion method with 100% sensitivity and specificity was the same as PCR method .CHROMagar™ MRSA medium had 100 sensitivity and 100% specificity.  Result of cefoxitin disk diffusion method with 100% sensitivity and specificity was the same as PCR method for detection of mecA gene. Cefoxitin disk diffusion method can be used as an alternative method of PCR for detection of MRSA.  

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Journal title

volume 17  issue 2

pages  14- 14

publication date 2023-03

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