Detection of Human Papillomavirus in Osophageal Squamous Cell Carcinoma samples in Mazandaran Province, Iran

Authors

  • Abdolvahab Moradi Department of Medical Virology, Golestan University of Medical Sciences, Gorgan, Iran
  • Alijan Tabarraei infectious diseases research center, Golestan University of Medical Sciences, Gorgan, Iran
  • Farzin sadeghi Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
  • Yousef Yahyapour Infectious Diseases & Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran
Abstract:

We examined 170 formalin-fixed paraffin-embedded samples from esophageal squamous cell carcinoma patients. All subjects live in Mazandaran province, a region with high incidence rate of esophageal cancer and have become known as the “Asian Esophageal Cancer Belt”. Samples were tested for HPV-DNA by MY09/11 and Gp5+/6+ general primers using nested PCR. Of the 170 ESCC samples, 86 (50.6%) were male and 84 (49.4%) were female. The mean age of the subjects was 66.5±11.1 and ranged from 35 to 91 years. Totally, HPV-DNA was detected in 62 (36.5%) of the esophageal squamous cell carcinoma samples by HPV L1 consensus primers. Considering the location of esophagus specimens, of 62 positive samples, 16 (25.8%) samples were in the upper third, 28 (45.2%) in the middle third, and 18(29.0%) in the lower third.  The current study showed a relatively substantial prevalence of HPV infection in esophageal squamous cell carcinoma samples in Mazandaran province.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

detection of human papillomavirus in osophageal squamous cell carcinoma samples in mazandaran province, iran

methods: we examined 170 formalin-fixed paraffin-embedded samples from esophageal squamous cell carcinoma patients. all subjects live in mazandaran province, a region with high incidence rate of esophageal cancer and have become known as the “asian esophageal cancer belt”. samples were tested for hpv-dna by my09/11 and gp5+/6+ general primers using nested pcr. results: of the 170 escc samples, ...

full text

Detection of Human Papillomavirus DNA by PCR in Esophageal Squamous Cell Carcinoma from Turkmen Sahra, North-East of Iran

Human papillomavirus (HPV) DNA has been identified in esophageal carcinomas. However, the incidence of HPV varies significantly in different geographical locations. In this study, neoplasms from Turkmen Sahra, a region in Golestan province in northeast part of Iran, with a high incidence of squamous cell carcinoma were analyzed for the presence of HPV DNA. Turkmen Sahra is located in the cancer...

full text

Prevalence of Squamous Cell Carcinoma in Mazandaran, Iran 2016-18

Background and purpose: Cancer is highly prevalent in modern societies and approximately one in three or four people is diagnosed with cancer. Oral cancer is one of the 10 primary causes of mortality due to late detection for many reasons, including being asymptomatic in initial stages, clinical presentation similar to other lesions, and variation in clinical manifestations. This study aimed at...

full text

Role of the Human Papillomavirus Infectio in Esophageal Squamous Cell Carcinoma

Background and Objective: Human papillomavirus (HPV) is one of the possible etiologic factors  in development of esophageal squamous cell carcinoma (ESCC). In this study we aimed to study the  role of HPV in ESCC. Patients and Methods:In this study, 140 cases of ESCC were analyzed for the HPV DNA by  polymerase chain reaction (PCR) using GP5+/GP6+ primers for L1 open reading frame (ORF) to  am...

full text

Human papillomavirus detection in head and neck squamous cell carcinoma

Squamous cell carcinomas of the head and neck (HNSCC) are a frequent diagnosis in anatomic pathology practice. Tobacco use and heavy alcohol consumption are known risk factors for HNSCC but in other cases human papillomavirus (HPV) is linked to carcinogenesis. HPV proteins E6 and E7 promote oncogenesis by blocking the action of p53 and pRB, respectively. An absence of p53 mutations in addition ...

full text

Human papillomavirus in oesophageal squamous cell carcinoma.

Thirty seven cases of oesophageal squamous cell carcinoma were studied by applying DNA slot blot analysis and in situ hybridisation using type specific probes for HPV 6, 11, 16 and 18. Cases of condyloma accuminata, cervical carcinoma, and laryngeal papilloma were used as controls. Blocks including areas of invasive carcinoma, intraepithelial neoplasia, and normal epithelium were studied in eac...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 7  issue 1

pages  741- 747

publication date 2017-06-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023