Detection of CYP2C18 m1, and m2 Alleles within an Iranian Population (Mazandaran) Using Denaturing High-Performance Liquid Chromatography (DHPLC)

Background: Genetic polymorphisms of cytochrome p450 in humans are the main cause of differences in the metabolism. The allele and genotype frequencies of CYP2C19 and CYP2C9 have been studied in some Iranian populations. The aim of present study was to examine the frequencies of CYP2C18m1, and CYP2C18m2, alleles in the Mazandarani ethnic group among Iranian Population. Materials and Methods: In this study, genomic DNA was extracted from leucocytes of one hundred unrelated healthy volunteers. The prevalence of the common variants CYP2C18 m1 and m2 alleles were studied by using high fidelity polymerase chain reaction (HF-PCR) - DHPLC methods. Results: The frequency of CYP2C18 m1 and m2 alleles were 0.0% and 3.0%, respectively. CYP2C18 genotypes wt/wt, wt/m1, wt/m2, m1/m1, m1/m2, and m2/m2 frequencies were 97 %, 0.0 %, 3.0%, 0.0 %, 0.0%, and 0.0%, respectively. Conclusion: The result of the current study shows that impaired CYP2C18 activity in 03% of our sample population may decrease extra hepatic metabolism of some important drugs such as Phenytoin. It may also affect transdemal delivery of drug substrate for this isoenzyme.