Design and Fabrication of a Novel Transplant Combined with Human Bone Marrow Mesenchymal Stem Cells and Platelet-rich Fibrin: New Horizons for Periodontal Tissue Regeneration after Dental Trauma

Authors

  • Azade Rafiee Postgraduate Student, Department of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
  • Hamid Moradian Department of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
  • Maryam Ayatollahi Transplant Research Center, Shiraz university of medical sciences, Shiraz, Iran; Shiraz Stem cell institute for cell therapy and regenerative medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:

Avulsed teeth that are replanted dried are more prone to loss. Recent tissue engineering studies focus onfabrication of various cell delivery systems to improve the overall prognosis of such teeth. To evaluate this newcell transplant method, we initially aimed at designing of PRF scaffold and determining BMMSCs viabilityand function on the fabricated scaffold. To test this concept in-vitro, human BMMSCs were isolated andcharacterized by cell surface marker, and their potential on osteogenic/adipogenic differentiation. The biologicaleffects of PRF scaffold on human BMMSCs were then investigated by cell proliferation assay. The data werequantified for statistical analysis. It was found that PRF significantly induced BMMSCs proliferation throughoutthe incubation period due to its growth factor secretion. Results of MTT assay showed an increasing trend duringthe testing period from day 1 to day 7. The result of scanning electron microscopy showed that BMMSCs couldtightly adhere to fibrin scaffold just shortly after seeding. These data suggest that the BMMSCs/PRF constructhas the potential to improve the clinical prognosis of replanted avulsed teeth in future. Additional studies areneeded to be performed before its clinical use.

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Journal title

volume 16  issue 4

pages  1370- 1378

publication date 2017-11-01

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