Decreased Expression of Arginine-Phenylalanine-Amide-Related Peptide-3 Gene in Dorsomedial Hypothalamic Nucleus of Constant Light Exposure Model of Polycystic Ovarian Syndrome

Authors

  • Amin Ramazani Department of Medical Biotechnology, School of Advanced Medical Sciences and Technology, Shiraz University of Medical Sciences, Shiraz, Iran
  • Farhad Rahmanifar Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
  • Somayeh Ahmadloo Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Zahra Shaaban Department of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, Iran
Abstract:

Objective An abnormality in pulse amplitude and frequency of gonadotropin releasing hormone (GnRH) secretion is the most characteristics of polycystic ovarian syndrome (PCOS). On the other hand, arginine-phenylalanine-amide (RFamide)-related peptide-3 (RFRP3) inhibits the secretion of GnRH in mammalian hypothalamus. Therefore, the current study evaluated the expression of RFRP3 mRNA in dorsomedial hypothalamic nucleus (DMH) after induction of PCOS in a rat model of constant light exposure considering the possible role of parity on occurrence of PCOS. MaterialsAndMethods In the present experimental study, female nulliparous (n=12) and primiparous (n=12) rats were randomly subdivided into control and PCOS subgroups (n=6). PCOS were induced by 90 days exposure to constant light. After 90 days, blood, brain, and ovaries were sampled. Serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone were evaluated. In addition, six adult female ovariectomized rats as a control of real-time PCR tests were prepared and in the DMH of all rats, the relative mRNA expression of RFRP3 was assessed. Results Histological evaluation of ovaries represented the polycystic features. In addition, serum concentrations of testosterone in the PCOS subgroups were more than the controls (P

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Journal title

volume 12  issue 1

pages  43- 50

publication date 2018-06-01

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