Cytotoxic Effect of Capparis spinosa L. on the PLC/PRF/5 Human Hepatocellular Carcinoma Cell Line
Authors
Abstract:
ABSTRACT Background and Objectives: Capparis spinosa has been used in traditional medicine for various applications including treatment of liver disorders and cancer. We studied the effects of this plant on cell proliferation and morphological characteristics of PLC/PRF/5 liver cancer cell line. Methods: After preparing ethanolic extract of the plant, the inhibitory effect of the extract was assessed using MTT assay, and morphological changes were assessed by an inverted microscope. Results: C. spinosa ethanolic extract exhibited anti-cancer effects in a concentration-dependent manner. Half-maximal inhibitory concentration of the extract was 1051±4.21 mg/mL. Morphological changes including cell shrinkage, reduction of cell volume and nuclear condensation confirmed the inhibitory effect of C. spinosa on PLC/PRF/5 cells. Conclusion: According to the results of this study, extract of C. spinosa seems to be suitable for prevention and treatment of liver cancer. Further studies on animal models could verify the efficiency of the extract against cancer cells. KEYWORDS: Plants, Medicinal, Capparis spinosa, Liver Neoplasms.
similar resources
Cytotoxicity effect of Capparis spinosa L. on the HepG2 human hepatocellular carcinoma cell line
Masoud Sheikh, Hamid Reza Eshraghi, Masoud Khoshnia, Masoumeh Mazandarani & Abdolvahab Moradi* Department of Veterinary Basic Science, College of Veterinary Science, Science and Research branch, Islamic Azad University, Tehran, Iran; Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran; Department of Botany, Gorgan Branch, Islamic Az...
full textCytotoxic Effect of Boswellia Serrata Hydroalcholic Extract on Human Cervical Carcinoma Epithelial Cell Line
Abstract Background and Objective: Cervical cancer is the second most common cancer in women. Boswellia serrata is a medicinal herb with anticancer, antibacterial, antiulcer, antifungal properties. Since the antitumor effect of this medicine has not been studied on cancer cell lines, we aimed to investigate the antitumor effect of Boswellia serrata on cervical cancer cell lines. Material ...
full textStudy of cytotoxic properties of Rosa damascena extract in human cervix carcinoma cell line
Objective: Cancer is a major health problem worldwide and current therapies for cancer are often limited by short-term efficacy due to drug resistance. There has been much interest in the use of naturally occurring compounds with chemo-preventive and chemotherapeutic properties in the treatment of cancers. Rose is one of the most important groups of ornamental plants which their fruits and flo...
full textthe cytotoxic effects of cadmium chloride on the human lung carcinoma (calu-6) cell line
cadmium has been known to be harmful to human health, mainly via contaminated drinking water, food supplies, tobacco and industrial pollutants. the aim of this study was to determine the ability of cadmium chloride (cdcl 2 ) to cause cell death in the human lung carcinoma cell line (calu-6). the cells were grown in rpmi-1640 medium supplemented with 10% fcs, penicillin/streptomycin (100 u/ml, 1...
full textCytotoxic effect of pyocyanin on human pancreatic cancer cell line (Panc-1)
Objective(s): Pyocyanin, a blue-green pigment produced by Pseudomonas aeruginosa, interferes with host redox cycles, which can lead to generation of reactive oxygen species and progressive cellular oxidative damage. The aim of this study was to assess the influence of pyocyanin on human pancreatic cancer cell line.Materials and Methods: Polymerase Chain Reaction (PCR) was applied to confirm the...
full textEvaluation of Radiosensitive Effect of Tolmetin in Radiotherapy on Human Colonic Carcinoma Cell Line HT-29
Background: Radiotherapy is one of the cancer treatment methods. Although radioresistance and normal tissue toxicity limit the radiation therapy in certain anatomical locations, using some substances can be useful to increase radiosensitivity on cancer cells without cytotoxicity effect on normal cells. Objective: This study aimed to evaluate the radiosensitizing effect of tolmetin in radiother...
full textMy Resources
Journal title
volume 11 issue 4
pages 9- 12
publication date 2017-08
By following a journal you will be notified via email when a new issue of this journal is published.
No Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023