Comparative attenuation of African horsesickness virus in mosquito (Aêdes albopictus) and in hamster kidney (BHK-21) celllines

Characteristics of Sindbis virus temperature-sensitive mutants in cultured BHK-21 and Aedes albopictus (Mosquito) cells.

A number of the temperature-sensitive mutants of Sindbis virus originally isolated and characterized by Burge and Pfefferkorn (1966, 1968) were reexamined for their abilities to grow and complement one another in cultured BHK-21 and Aedes albopictus (mosquito) cells. The response of the mutants to conditions of high and low temperature was similar in cultured cells of both the vertebrate and in...

Growth of chikungunya virus in baby hamster kidney cell (BHK-21-clone 13) suspension cultures.

This report describes the methods used to obtain high titers of chikungunya virus with suspension cultures of BHK-21-clone 13 cells. The cells were grown at 37 C to a cell concentration of 10(6) to 2 x 10(6) per ml. After maximum cell growth, the cells were inoculated with chikungunya virus at a multiplicity of 1 to 2 50% suckling mouse intracerebral lethal doses (SMICLD(50)) per cell in the sp...

Growth and persistent infection of African horse-sickness virus in a mosquito cell line

Preparation and characterization of a soluble precipitating antigen from African horsesickness virus propagated in cell cultures

Ultrastructure of microfilament bundles in baby hamster kidney (BHK-21) cells. The use of tannic acid

After standard glutaraldehyde-osmium tetroxide fixation procedures, the majority of microfilament bundles in BHK-21 cells exhibit relatively uniform electron density along their long axes. The inclusion of tannic acid in the glutaraldehyde fixation solution results in obvious electron density shifts along the majority of microfilament bundles. Striated patterens are frequently observed which co...

Comparative studies on the serological responses of horses to African horsesickness virus