Cloning of Rotavirus Outer Capsid Protein (VP7) Gene into the pGEM Vector
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Cloning of Rota Virus Outer Capsid Protein (VP7) Gene into the pGEM Vector
Background and Aims: In humans the group A rotaviruses are associated with endemic diarrhea in children under the age of 5, leading to approximately 800,000 deaths every year. Introduction of rotavirus vaccines into childhood immunization programs can contribute to substantial reduction in mortality from rotavirus gastroenteritis in developing countries and virtually eliminating hospitalization...
full textcloning of rota virus outer capsid protein (vp7) gene into the pgem vector
background and aims: in humans the group a rotaviruses are associated with endemic diarrhea in children under the age of 5, leading to approximately 800,000 deaths every year. introduction of rotavirus vaccines into childhood immunization programs can contribute to substantial reduction in mortality from rotavirus gastroenteritis in developing countries and virtually eliminating hospitalization...
full textTwo proline residues are essential in the calcium-binding activity of rotavirus VP7 outer capsid protein.
Rotavirus maturation and stability of the outer capsid are calcium-dependent processes. It has been shown previously that the concentration of Ca2+-solubilizing outer capsid proteins from rotavirus particles is dependent on the virus strain. This property of viral particles has been associated with the gene coding for VP7 (gene 9). In this study the correlation between VP7 and resistance to low...
full textCross-linking of rotavirus outer capsid protein VP7 by antibodies or disulfides inhibits viral entry.
Antibodies that neutralize rotavirus infection target outer coat proteins VP4 and VP7 and inhibit viral entry. The structure of a VP7-Fab complex (S. T. Aoki, et al., Science 324:1444-1447, 2009) led us to reclassify epitopes into two binding regions at inter- and intrasubunit boundaries of the calcium-dependent trimer. It further led us to show that antibodies binding at the intersubunit bound...
full textrecombinant outer capsid glycoprotein (vp7) of rotavirus expressed in insect cells induces neutralizing antibodies in rabbits
background: rotaviruses cause diarrhea in infants and young children worldwide. rotavirus outer capsid protein, vp7 is major neutralizing antigen that is important component of subunit vaccine to prevent rotavirus infection. many efforts have been done to produce recombinant vp7 that maintain native characteristics. we used baculovirus expression system to produce rotavirus vp7 protein and to s...
full textRecombinant Outer Capsid Glycoprotein (VP7) of Rotavirus Expressed in Insect Cells Induces Neutralizing Antibodies in Rabbits
BACKGROUND Rotaviruses cause diarrhea in infants and young children worldwide. Rotavirus outer capsid protein, VP7 is major neutralizing antigen that is important component of subunit vaccine to prevent rotavirus infection. Many efforts have been done to produce recombinant VP7 that maintain native characteristics. We used baculovirus expression system to produce rotavirus VP7 protein and to st...
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Journal title
volume 2 issue None
pages 34- 38
publication date 2008-02
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