Cloning and sequence analysis of VP1, VP2 and VP3 genes of Indian chicken anemia virus
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Abstract:
Chicken anemia virus was detected by PCR in tissue samples collected from poultry flocks in Gujarat,India. The VP1, VP2 and VP3 gene sequences of CAV from Anand, Gujarat were obtained after cloning thePCR products in pDrive cloning vector. Nucleotide sequence alignment with other CAV sequencesdemonstrated overall identity of 95-98.8%, 98.8-99.8% and 98.8-100% for VP1, VP2 and VP3 regions,respectively. Deduced amino acid sequences revealed 91.7-99.7%, 99-100% and 97.3-100% homologies forVP1, VP2 and VP3 proteins, respectively, indicating high level of genome conservation. Further, placementof critical nucleotides and amino acids at particular positions indicated that Anand CAV is possibly of morepathogenic potential. The CAV isolates were phylogenetically grouped together independent of theirgeographic origin.
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Journal title
volume 14 issue 4
pages 354- 357
publication date 2013-12-30
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