Chemical composition and biological activities of methanolic extract of Scrophularia oxysepala Boiss

Isolated five known iridoids glycosides (Scrophuloside A, Harpagoside B, 5-hydroxyloganin, 8-O-acetylharpagide and 6-O-methyl,1-glucopyranosyl catalpol ),one phenyl ethanoid glycoside (Verbascoside) and a phenyl ethanol amine (2-(4-Chlorobenzyl amino) ethanol ) compound from the methanolic extract of aerial parts of Scrophularia oxysepala using by high performance liquid chromatography based on isocratic and liner gradients by C18 column . The structure elucidations of the isolated compounds were performed by spectroscopic methods include 1H-NMR, 13C-NMR, 2 D NMR technique such as HMBC ( in deuterated methanol as solvent) , GC-MS and UV also methanolic extract and fractions ( fractionated on solid phase extraction on C18 cartridge(Spack-C18)) of this plant was tested for free radical scavenging properties toward the 1, 1-diphenyl-1-picrylhydrazyl (DPPH), general toxicity (Brine shrimp toxicity assay) , insecticidal ( Contact toxicity insecticidal assay) and antimalarial activities (hemebiocrystallization inhibition assay).