Characterization of Miconazole effects on Mice fetus liver tissue using FTIR-MSP
Authors
Abstract:
Azole agents especially Miconazole are widely used even during pregnancy as antifungal agents. The aim of this study was to assess the usefulness of FTIR Micro-Spectroscopy for discriminating of Miconazole treated liver tissue from control liver tissue. The mice were injected with Miconazole (60 mg/Kg) on gestation day 9 and they were dissected on pregnancy day 15. The fetus fixed, dehydrated and embedded in paraffin. Sections of liver (10 μm) were prepared from control and treated fetus groups by Microtome and deparaffinized with xylene. The spectra were collected using FTIR-MSP in the region of 4000-400 cm-1. All spectra were normalized to amide II band (1454cm-1) after baseline correction of entire spectrum. The results were shown by 2nd derivatization of spectra and also subtracting from control spectra. Miconazole induces some minor changes in the mouse fetus liver at cellular levels when mother is exposed. The most important calculated alterations are in the production of fetus liver proteins. α helical and β sheet structures have shown significant variations, indicating protein alterations configurationally
similar resources
Characterization of Miconazole Effects on Mice Fetus Liver Tissue Using FTIR-MSP
Azole agents especially Miconazole are widely used even during pregnancy as antifungal agents. The aim of this study was to assess the usefulness of FTIR Micro-Spectroscopy for discriminating of Miconazole treated liver tissue from control liver tissue. The mice were injected with Miconazole (60 mg/Kg) on gestation day 9 and they were dissected on pregnancy day 15. The fetus fixed, dehydrated, ...
full textFTIR Determination of Miconazole Effects on Mice Fetus Brain Tissue
Miconazole is an imidazole antifungal agent, commonly applied topically to the skin or mucous membranes. The aim of this study was to examine the alternative method for gaining mechanism or the bimolecular changes caused by the possible teratogenic effects of Miconazole on mice fetus brain tissue using FTIR-Microspectroscopy. The mice were injected with Miconazole (60 mg/Kg) on gestation day 9....
full textftir determination of miconazole effects on mice fetus brain tissue
miconazole is an imidazole antifungal agent, commonly applied topically to the skin or mucous membranes. the aim of this study was to examine the alternative method for gaining mechanism or the bimolecular changes caused by the possible teratogenic effects of miconazole on mice fetus brain tissue using ftir-microspectroscopy. the mice were injected with miconazole (60 mg/kg) on gestation day 9....
full textFTIR-Microspectroscopy Detection of Metronidazole Teratogenic Effects on Mice Fetus
Metronidazole is used to treat trichomoniasis, bacterial vaginosis, and other diseases. There are controversy aspects about its teratogenicity. A teratogenic agent can alter morphology or subsequent function of the fetus. The aim of this study was to examine an alternative method for the recognition of the mechanism or the bimolecular potential changes in mice fetus caused by Metronidazole usin...
full textFTIR-Microspectroscopy Detection of Metronidazole Teratogenic Effects on Mice Fetus
Metronidazole is used to treat trichomoniasis, bacterial vaginosis, and other diseases. There are controversy aspects about its teratogenicity. A teratogenic agent can alter morphology or subsequent function of the fetus. The aim of this study was to examine an alternative method for the recognition of the mechanism or the bimolecular potential changes in mice fetus caused by Metronidazole usin...
full textMy Resources
Journal title
volume 16 issue 2
pages 677- 684
publication date 2017-06-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023