Blockade of the Naloxone-induced Aversion in Morphine-conditioned Wistar Rats by L-Arginine Intra-central Amygdala

Authors

  • Abbas Haghparast Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Farzaneh Sabouni National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
  • Mahnaz Rahimpour MS student, Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
  • Manizheh Karami Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
  • Mohammad Reza Jalali Department of Pathology, School of Medicine, Shahed University, Tehran, Iran
  • Sara Karimi MS student, Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
Abstract:

Objective(s) Single injection of naloxone, a selective antagonist of morphine, prior to the drug conditioning testing was used to investigate on morphine dependence. Materials and Methods Conditioning to morphine (2.5-10 mg/kg, s.c.) was established in adult male Wistar rats (weighing 200-250 g) using an unbiased procedure. Nitric oxide agents were microinjected into the central amygdala prior to naloxone-paired place conditioning testing. Results The results showed that morphine produced a significant dose-dependent place preference in animals. Naloxone (0.1-0.4 mg/kg, i.p.) injections pre-testing of the response to morphine (7.5 mg/kg, s.c.) caused a significant aversion at the higher doses (0.4 mg/kg, i.p.). This response was reversed by microinjection of L-arginine (0.3-3 μg/rat, intra-central amygdala) prior to naloxone on the day of the testing. The response to L-arginine was blocked by pre-injection of NG-nitro-L-arginine methyl ester (L-NAME) (intra-central amygdala). Conclusion A single injection of naloxone on the test day of morphine place conditioning may simply reveal the occurrence of morphine dependence in rats, and that the nitric oxide in the central amygdala most likely plays a key role in this phenomenon.

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blockade of the naloxone-induced aversion in morphine-conditioned wistar rats by l-arginine intra-central amygdala

objective(s) single injection of naloxone, a selective antagonist of morphine, prior to the drug conditioning testing was used to investigate on morphine dependence. materials and methods conditioning to morphine (2.5-10 mg/kg, s.c.) was established in adult male wistar rats (weighing 200-250 g) using an unbiased procedure. nitric oxide agents were microinjected into the central amygdala prior ...

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Reversal Effect of Intra-Central Amygdala Microinjection of L-Arginine on Place Aversion Induced by Naloxone in Morphine Conditioned Rats

Background: Role of nitric oxide (NO) on expression of morphine conditioning using a solely classic task has been proposed previously. In this work, the involvement of NO on the expression of opioid-induced conditioning in the task paired with an injection of naloxone was investigated. Methods: Conditioning was established in adult male Wistar rats (weighing 200-250 g) using an unbiased procedu...

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reversal effect of intra-central amygdala microinjection of l-arginine on place aversion induced by naloxone in morphine conditioned rats

background: role of nitric oxide (no) on expression of morphine conditioning using a solely classic task has been proposed previously. in this work, the involvement of no on the expression of opioid-induced conditioning in the task paired with an injection of naloxone was investigated. methods: conditioning was established in adult male wistar rats (weighing 200-250 g) using an unbiased procedu...

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Reversal effect of intra-central amygdala microinjection of L-arginine on place aversion induced by naloxone in morphine conditioned rats.

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Journal title

volume 14  issue 2

pages  167- 176

publication date 2011-03-01

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