Bioaccumulation of copper in Hepatopancrease and Muscle tissues of shrimp Litopenaeus vannamei after exposure to synthesized CuO Nanoparticles

This paper intends to study the accumulation of copper oxide nanoparticles (CuO NPs) on hepatopancreas and muscle tissues in Shrimp L. vannamei. The CuO NPs were synthesized through chemical sequestration and were studied by precise techniques of FTIR, SEM, AFM, XRD, and DLS, and then their accumulation on L. vannamei were evaluated. In this research, the bioaccumulation of CuO particles on hepatopancreas and muscles tissues were studied after exposure to three concentrations (0.25, 0.5 and 1 mg/L) of CuO NPs. According to the findings of this study, the average copper concentrations in these three treatments (0.25, 0.5 and 1 mg/L) were 18.676, 18.585, and 18.484 mg/Kg respectively in muscle tissues and in the same treatments of copper concentration in hepatopancreas tissues, were 15.624, 15.351 and 14.250 mg/Kg, respectively. The results showed that copper concentration in muscles tissues was higher than in hepatopancreas tissues, in 1 mg/L treatment this difference was statistically significant (p˂0.05). There was no much difference among  copper concentrations in the muscle tissue and there was no significant differences. Increase of CuO NPs concentrations in different treatments was associated with decrease of copper concentrations in hepatopancreas tissue. Showing reverse correlation of these parameters(r = -0.45, p˂0.05). Therefore, according to the results of this study, the mean copper concentrations in these two tissues were  lower than the standards of the World Health Organization, which may be considered as unlikely to endanger the human life.