B-Glucan contents in calli of Oryza sativa L. var Hashemi under different nutritional treatments . Golnaz Tajadod1*, Mozhgan Farzami Sepehr2, Zeinab Kalami1
Authors: not saved
Abstract:
Studies show that Oryza sativa L. contains a structure named β-glucan with anti cancer properties. In the present study, a tissue culture for rice was carried out and β-glucan content was measured under various sugar and vitamin levels in basic MS medium. Seeds of Hashemi cultivar were cultured on MS medium. Two plant hormones, namely, 2,4-D (0, 1, 1.5, 2 and 3 mg/l) and kinetin (0,0.5, 1, and 1.5 mg/l) were used to form callus after one week. To extract β-glucan, calluses were incubated by three different sugar treatments with concentrations 15, 30, and 45 g/l and by three vitamin treatments with concentrations 2/5, 5, and 10 cc. After 30 days, those calluses treated with sucrose 45 g/l had a deeper yellow color than other calluses. In fact, higher concentrations of sugar led to deeper color of calluses which were more rigid and dry and, at the same time, more fragile and less bright in color. The calluses were then dried in an oven, and soluble and insoluble sugars were extracted. Results indicated that highest levels of soluble and insoluble sugars (including β-glucan) were achieved in medium containing 3% sucrose. Increase in vitamins did not generally affect soluble and insoluble sugars levels, and just in the medium containing 5cc vitamin, insoluble sugars increased.
similar resources
تاثیر میدان مغناطیسی بر خصوصیات بیوشیمیایی بذر برنج رقم هاشمی Oryza sativa L. (var. Hashemi)
full text
Determination of Residual Contents of Pesticides in Rice (oryza Sativa L.) Crop from Different Regions of Pakistan
The study was conducted to investigate the pesticide residues in unhusked and husked rice (Oryza sativa L.) on Basmati-385 and Irri-6 varieties in major rice growing areas of Punjab, Pakistan. The concentration of four pesticides was determined by gas chromatography with flame ionization detection (GC-FID). In all the 400 samples, four pesticides, i.e., Karate, Malathion, Novacran and Padan wer...
full textInfluence of cooking on anthocyanins in black rice (Oryza sativa L. japonica var. SBR).
The composition and thermal stability of anthocyanins in black rice (Oryza sativa L. japonica var. SBR) produced in California were investigated. Six anthocyanin pigments were identified and quantified by high performance liquid chromatography using photo diode-array detection (HPLC-PDA) and electrospray ionization mass spectrometry [LC-(ESI)MS/MS]. The predominant anthocyanins are cyanidin-3-g...
full textEffect of potassium fertilizers on chlorophyll florescence and photosynthetic pigments in rice (Oryza sativa L. var. Tarom Hashemi) in regulated deficit irrigation condition
In order to evaluate the effect of deficit irrigation and different types of potassium fertilizers on improving the photosynthetic pigments content and chlorophyll florescence parameters in rice (cv. Tarom Hashemi), two field experiments were conducted in the Rice Research Institute (Amol) and model farm located in Savadkouh in a split plot based randomized complete block design with three repl...
full textCorrelation Studies in Rice (Oryza sativa L.)
Seventy genotypes of rice (Oryza sativa L.) were evaluated during Kharif 2012 to study the nature and extent of correlation among yield and yield attributing characters, days to 50 per cent flowering, days to maturity, number of effective tillers per plant, plant height, panicle length, number of grains per panicle, 1000-grain weight, grain yield per plant, kernel length, kernel breadth and L/B...
full textStudy of Genetical Correlation and Diversity of Morphological Traits Induced in Rice (Oryza sativa L.) Mutant Population of Hashemi Cultivar
Mutagenesis has been one of the important sources of genetic diversity and Plant mutants can be important bio-resources for crop breeding and functional genomics studies. Breeding conventional methods for generating of genetic variability are of low efficiency. We showed that treatment of seeds of rice(Hashemi cultivar) with 0.8% EMS for 8 h caused visible phenotypic variations on M2 rice mutan...
full textMy Resources
Journal title
volume 2 issue 3
pages 471- 475
publication date 2012-04-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023