Association of CXCL12 and CXCR4 Genes Polymorphism with Proliferative Diabetic Retinopathy in Diabetic Patients

Authors

  • Aghaei, Shiva Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • Farashahi Yazd, Ehsan Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
  • Gharibi, Saba Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
  • kolahdouz, Parisa Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
Abstract:

Introduction: Patients with diabetes are prone to develop complications related to the retina. So far, several genes have been reported in connection with the progression of retinopathy, and a group of these genes is genes involved in angiogenesis. CXCL12 and CXCR4 genes are among the most important genes in this path, which have been less studied and investigated. The aim of this study was to determine the relationship between polymorphism of CXCL12 (rs1801157) and CXCR4 (rs2228014) genes and the risk of proliferative diabetic retinopathy in the population of Yazd City. Methods: In the present case-control study, rs2228014 and rs1801157 polymorphisms were investigated among 103 patients with diabetes in two groups, case and control. In this study, the control group included 49 diabetic patients who did not show retinopathy complications, and the case group consisted of 54 diabetic patients with proliferative retinopathy complications. The Genotyping of rs2228014 polymorphism was examined by ARMS-PCR method and rs1801157 variant by RFLP-PCR technique. The statistical analysis of data was conducted using SPSS software version 16 and chi-square test. Results: Our results represented no association among the rs1801157 and rs2228014 polymorphisms and the risk of proliferative diabetic retinopathy (P>0.05). Conclusion: This study did not confirm association of the rs1801157 and rs2228014 polymorphisms with proliferative diabetic retinopathy and further studies are required to investigate the correlation of polymorphisms of interest with PDR.  

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Journal title

volume 30  issue 7

pages  5032- 5041

publication date 2022-10

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