Assessment of Foot and Mouth Virus Subtype O2016 Genetic Alterations During Successive Passages in BHK Monolayer
author
Abstract:
Abstract : Foot and Mouth Disease is one of the important live stocks contagious viral disease caused by Aphtovirus genus , that is belong to family RNA virus picornaviride. The important characteristic of FMD virus is high mutation that give rise to diversity of Antigen in surface of Neutralizing proteins. For this reason FMD virus have 7 distinct serotype and many subtype. Vaccination is one of the methods for control disease in order to different type and subtype of FMD virus , the effective vaccine should have virus that closed relationship with field virus and also many passage on cell culture may cause mutation on viral genome . The rate of genetic changes of FMD virus type O2016 during of 70 passages in BHK monolayer cell is the main reasone of this paper . After determine the type of virus in epithelium sample by ELISA , the virus inoculated in BHK monolayer cell for 70 consequence passage . harvested viruses in 10 selected passage (P1,P10 , P20,P30,P40,P50,P60,P70) is done by RT-PCR , ELISA , titration , real time PCR, Double dimension virus neutralization test for immunological relationship value (r value) and Nucleotide sequencing of 1D segment of viral genome . harvested FMD virus type O2016 From passage 1 until 70 be constant and no significant change neither in Sequencing nor in r value .
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Journal title
volume 13 issue None
pages 0- 0
publication date 2019-12
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