Antioxidant Effects of Thyme (Thymus vulgaris) Extract on Ram Sperm Quality during Cryopreservation

Authors

  • E. Dirandeh Department of Animal Science, Faculty of Agriculture, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
  • M. Behroozlak Department of Animal Science, Faculty of Agriculture, Payame Noor University, Tehran, Iran
  • N. Hedayat Evrigh Department of Animal Science, Faculty of Agricultural Science, University of Mohaghegh Ardabili, Ardabil, Iran
  • V. Vahedi Department of Animal Science, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
Abstract:

Oxidative stress during freezing-thawing reduces sperm motility, viability, membrane functions and antioxidant capacity. Thymus vulgaris has antioxidant properties due to phenolic compounds. The purpose of current study was to evaluate the effect of Thymus vulgaris extract as a natural antioxidant on post-thawed Moghani ram sperm quality. Semen samples were collected twice a week from four rams. Samples were then pooled and diluted with Tris based extender supplemented with different concentrations of Thymus vulgaris (0, 2, 4, 8, 12 and 16 mL per dL diluent solutions). Following the cooling and equilibration stages for semen samples, the samples were frozen and stored in liquid nitrogen until evaluation. Following this freezing procedure, samples were thawed, and the sperm motility and velocity parameters, viability and plasma membrane integrity were evaluated. Our results showed that the addition of 2 and 4 mL/dL extracts at the extender stage increased the total motility percentages (P

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Journal title

volume 8  issue 2

pages  263- 269

publication date 2018-06-01

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