Ammonium to Nitrate Ratio Affects Protocorm Like Bodies PLB Formation In vitro of Hybrid Cymbidium

ammonium to nitrate ratio affects protocorm like bodies plb formation in vitro of hybrid cymbidium

carbon and nitrogen are the two elements that most affect plant organogenesis. in vitro, usually as part of the macronutrients. some anecdotal evidence from the literature suggests that the ammonium (nh4+) to nitrate (no3–) ratio may affect orchid organogenesis. in this study, to test this hypothesis, different nh4+: no3– ratios were tested on the development of protocorm-like bodies (plbs) of ...

Jasmonic Acid, but not Salicylic Acid, Improves PLB Formation of Hybrid Cymbidium

Increasing nitrate/ammonium ratio for improvement of garlic micropropagation

The effect of different culture media with different growth regulator and nitrate/ammonium ratios on split garlic shoots was studied to obtain an optimized micropropagation protocol. Three basal media: MS, BDS and BLM (i.e. BDS modi®ed to obtain a nitrate:ammonium ratio of 35 mM:8 mM) combined with two concentrations of NAA (0.5 or 5 mM) and BAP (1 or 10 mM) were evaluated in three Colorado gar...

Effects of organic additives and different carbohydrate sources on proliferation of protocorm- like bodies in Dendrobium

The purpose of this study was to investigate the effect of the addition of different organic additives and sugar types as carbon source on the growth and proliferation of protocorm-like bodies (PLBs) of a very new Dendrobium hybrid known as D. Alya Pink (DAP). PLBs were supplemented with homogenates of banana, tomato, and coconut water at various concentrations. Proliferation rate was recorded ...

Effect of Banana Extract on Growth and Development of Protocorm Like Bodies in Dendrobium sp. orchid

An experiment was carried out in Plant Tissue Culture Laboratory, Department of Crop Botany, Bangladesh Agricultural University, Mymensingh to investigate the effect of banana extract on micropropagation of Dendrobium sp. var. Sonia orchid through PLBs. The experiment was conducted during July 2012 to October 2013. Half-Murashige and Skoog (1/2MS) medium were used as basal medium and the medium...

Thidiazuron Triggers Morphogenesis in Rosa canina L. Protocorm-Like Bodies by Changing Incipient Cell Fate

Thidiazuron (N-phenyl-N'-1,2,3-thiadiazol-5-ylurea; TDZ) is an artificial plant growth regulator that is widely used in plant tissue culture. Protocorm-like bodies (PLBs) induced by TDZ serve as an efficient and rapid in vitro regeneration system in Rosa species. Despite this, the mechanism of PLB induction remains relatively unclear. TDZ, which can affect the level of endogenous auxins and cyt...