Acetylcholinesterase Inhibitory Activity of Standardized Cannabinoids-rich Fractions
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Abstract:
Background: Since cannabis has been legally allowed for medicinal purposes in many countries, it has become more interesting issue, particularly in neurologic disorders such as Alzheimer’s disease (AD). Inhibition of acetylcholinesterase (AChE) is one of the mechanisms for the treatment of AD. Objectives: The present study aimed to establish a method for preparation of cannabinoids-rich extracts and determined their AChE inhibitory activity. Methods: The cannabinoids-rich extracts were prepared through a green extraction process using microwave-assisted extraction (MAE) followed by hydrophobic column separation. The contents of cannabidiol (CBD) and Δ-9-tetrahydrocannabinol (THC) were determined using high-performance liquid chromatography (HPLC). In vitro AChE inhibitory activity was determined via photometric method using AChE from Electrophorus electricus. Results: Three cannabinoids-rich fractions were obtained with different concentrations of CBD and THC, namely Fractions I (CBD of 8.1% w/w; THC of 52.2% w/w), II (CBD of 9.2% w/w; THC of 8.0% w/w) and III (CBD 1.3% w/w, THC 33.5% w/w). These cannabinoids-rich extracts exhibited AChE inhibitory activity, with the IC50 values of 52.3, 59.8 and 71.2 µg/mL, respectively. Conclusion: This finding suggests that CBD, but not THC, might be an active compound contributing to AChE inhibitory effect.
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Journal title
volume 9 issue 1
pages 0- 0
publication date 2023-12
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