16S-Amplified Ribosomal DNA Restriction Analysis Assay for Discriminating Potentially Probiotic Lactobacillus Species Isolated from Traditional Dairy Products

Authors

  • Abolfazl Barzegari Ph.D. Candidate of Medical Biotechnology, Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran
  • Davoud Afshar Assistant Professor of Medical Bacteriology, Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
  • Mohammad Amin Hejazi Associate Professor of Food Biotechnology, Department of Food Biotechnology, Branch for Northwest & West region, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Tabriz, Iran
  • Solat Eslami Assistant Professor of Medical Biotechnology, Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran
  • Soudeh Ghafouri-Fard Associate Professor of Medical Genetics, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract:

Background: Traditional dairy products are the main sources for probiotic bacteria. This study aimed to isolate and characterize the potentially probiotic Lactobacillus strains isolated from traditional dairy products in Iran. Methods: Microbial population of each dairy product was enriched and screened for acid- and bile- resistant strains. The isolates were primarily characterized by conventional phenotypic and biochemical tests. The species were finally identified using restriction analysis and 16S rDNA sequencing. In addition, the antibacterial activity of the four most common species was evaluated against seven gastrointestinal pathogens. Results: From a total of nine yogurt and cheese samples, 17 acid-resistant Lactobacillus strains were isolated (survival>50% in PBS buffer, pH 2.5 for 3 h). Eight of them also showed high tolerance to bile. Also, the superiority of molecular techniques over phenotypic tests for effective identification of species was observed. Biochemical tests were useful in combination with 16S-amplified ribosomal DNA restriction analysis (ARDRA) technique to discriminate the isolates at species level. Conclusion: 16S–ARDRA in combination with biochemical tests is suggested as a potentially useful method for accurate identification of Lactobacillus species where the 16S-rDNA sequencing could not discriminate between two closely related species. Considering the acid and bile resistance capacity of isolated strains, traditional dairy products may be used as important sources of probiotic strains.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Screening of Potentially Probiotic Lactobacillus Possessing Surface Layer Protein from Iranian Traditional Dairy Products

One of the most commonly observed surface structures on the prokaryotic cell envelopes is monomolecular crystalline array of proteinaceous subunits termed Surface Layers or S-layers. Due their self assembly ability and the highly ordered, regular structure down to the nanometer scale, S-layers were demonstrated to possess a great potential for nanobiotechnological applications. Some probiotic b...

full text

In Vitro Study of Potentially Probiotic lactic Acid Bacteria Strains Isolated From Traditional Dairy Products

BACKGROUND Probiotic microorganisms are selected based on their long history of use as well as their lack of side effects. Nowadays, the consumption of probiotic products is growing intensively in developing countries. Researchers who work in the food industry and research centers pay more attention to the identification of new probiotic bacteria with better performance characteristics as well ...

full text

Identification of clinical isolates of actinomyces species by amplified 16S ribosomal DNA restriction analysis.

Amplified 16S ribosomal DNA (rDNA) restriction analysis (ARDRA), using enzymes HaeIII and HpaII, was applied to 176 fresh and 299 stored clinical isolates of putative Actinomyces spp. referred to the Anaerobe Reference Unit of the Public Health Laboratory Service for confirmation of identity. Results were compared with ARDRA results obtained previously for reference strains and with conventiona...

full text

in vitro study of potentially probiotic lactic acid bacteria strains isolated from traditional dairy products

conclusions there are many of different species of lactobacillus probiotics in traditional dairy products of the markazi province, based on the findings of this study. it is recommended for researchers to isolate these strains and investigate their probiotic characteristics in order to reproduce them for use in food production as well as for medical treatment. results nineteen gram-positive and...

full text

Probiotic Properties of Lactobacillus Species Isolated from Local Traditional Fermented Products

The present study aims to isolate Lactobacillus species from locally fermented vegetables and to characterize selected isolates for their probiotic potential. Seventeen Lactobacillus strains (9 Lactobacillus plantarum 1, 3 Lactobacillus pentosus, 2 Lactobacillus brevis 1, 2 Lactobacillus brevis 3 and 1 Lactobacillus salivarius) were isolated and tested for their probiotic potential. This includ...

full text

Amplified 16S ribosomal DNA restriction analysis for identification of Avibacterium paragallinarum.

A molecular technique based on the restriction fragment length polymorphism of the 16S ribosomal genes amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samples of broiler chickens, breeders, and laying h...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 25  issue 2

pages  175- 186

publication date 2018-03-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023