چند شکلی ژنتیکی4G/5G در پروموتور ژن مهارکننده فعال کننده پلاسمینوژن-1 در زنان با سابقه سقط‌های خود به خودی مکرر و افراد کنترل سالم

Background & Aims: Deletion/insertion of a single guanosine in the promoter of the plasminogen activator inhibitor-1 (PAI-1) gene, resulting in two alleles containing either 4 or 5 guanosines (G). Results of recent studies showed that presence of PAI-1 4G allele may increase the risk of human disease.  The aim of present study was to determine the 4G/5G genetic variation in the promoter of PAI-1 gene in patients with recurrent miscarriage and healthy controls. Materials and Methods: Genotypes of 45 cases with recurrent miscarriage (at least 3 unexplained abortions) and 64 controls were determined by RFLP-PCR.Results:  The findings of present study shows: Frequency (percent) of PAI-1 4G allele in cases and controls were 29(32.22%) and47(36.72%), respectively frequency (percent) of PAI-1 5G allele in cases and controls were 61(67.78%) and 81(63.28%),  respectively. Frequency (percent) of PAI-1 5G/5G and 4G/4G was 16(35.56%) and 29(64.44%) in cases and 17(26.56%) and 47(73.44%) in controls, respectively. PAI-1 4G/4G genotype was not observed in studied groups. Statistical analysis showed that the frequencies of PAI-1 -675 4G/5G alleles and genotypes did not   significantly differ between cases and controls. Conclusion: The findings of present study show that it is not possible to find any association between PAI-1 -675 4G allele and predisposition to recurrent miscarriage in tested population.  Source: Urmia Med J 2011: 22(2):165 ISSN: 1027-3727