سلول‌های IgM+CD5+ در مدل موشی سرطان با ردۀ سلولی BCL1

Background: Animal models are crucial for the development of new therapies, especially in serious diseases such as cancer. In this study, cancer (leukemia) was established by an injection of cancerous cells and followed by evaluating cell markers in spleen and blood.&nbsp; Materials and Methods: 5&times;106 BCL-1 cells were injected through tail vein into syngenic BALB/c mice. Then, 5 mice were killed after two weeks and 5 after four weeks and changes in spleen index, peripheral blood leukocytes and histology of organs were assessed. Spleen and blood cells were evaluated by flow cytometry using IgM and CD5 as markers of cancer cells.&nbsp; Results: Spleen index was increased in injected groups statistically significant (p<0.05) and the most increase was observed in spleen area of the 4-week group (about 1.5 fold of control group). Lymphocyte to the neutrophil ratio in peripheral blood smear was augmented from 4.9 to 8.8 (p<0.05). The percentage of IgM+CD5+ cells in the spleen increased from 2.5% in the control group to 14% in 2 weeks and 32% in 4-weeks group. In the histological examination of the spleens, the white pulp was developed.&nbsp; Conclusion: In this method, cancer cells were established in animals. Changes in spleen size, blood cells ratio and the percentage of IgM+CD5+ cells in spleen can be used to track the status of the establishment of cancer cells in animals and could be considered as indicators to use for research on new therapies.