تاثیر تمرین تداومی بر میزان پروتئینهای BAX و BCL-2 قلبی در رتهای مسموم شده با آب اکسیژنه

Introdution: The research has been indicated that reactive oxygen species induced the apoptosis of cardiomyocytes. However, this mechanism has been unclear. The aim of this study was to investigate the effects of concurrent training on some of the heart apoptosis variables  (BAX,BCL-2) following by the injection of different H2O2 dose in Wistar rat males. Methods: 50 male rats were randomly assigned to 5 groups with 10 rats in which group. Groups included: group (1): control group (C), group (2, 3): injection of 1 and 2ml H2O2,  group (4,5): exercise and injection of 1and 2 ml H2O2. Exercise groups have been run on treadmill for four days during 8 weeks  at moderate intensity. 24 hr after the last exercise and in anesthetic state all rats have been knockouted to determine bax and bcl2 proteins ratio. For measuring the BAX and BCL-2 proteins were used by ELISA technic and total protein were determined by brad ford technic. Data were analyzed by SPSS 17, one ways ANOVA was used to analysis of data at the level of p≤0/05. Results: The results have been indicated that after two month continues training  no significant difference in  BAX, BCL-2 proteins and BAX/BCL2 ratio in exercise and H2O2 groups in compared by control groups. Conclusion: based on the result of this study if the 8-week continues training has been followed by H2O2 injection with both of one and two ml H2O2 concentration, it may not  have induced apoptosis cardio myocyte in rats. And it may adjusted the synthesis and degradation myocardial