بررسی ژنوتایپینگ و مقاومت آنتی‌بیوتیکی سویه‌های اسینتوباکتربومانی جداشده از بیماران بستری در بیمارستان‌های آموزشی شهرکرد به روش Pulsed- Field Gel Electrophorsis

Introduction: Acinetobacters especially Acinetobacter baumannii causing nosocomial infections in hospitals intensive care units and can cause a variety of hospital infections such as bacteremia, meningitis, pneumonia and urinary tract infections. There are several molecular techniques for microbial genotyping, among them Pulsed- Field Gel Electrophoreses is introduced as the gold standard for sub typing of bacteria. The aim of this study  was investigating the molecular typing of A. baumannii strains with PFGE as well as the relationship between common types available and their antibiotic resistance. Methods: In this descriptive - analysis study, 50 Acinetobacter baumannii were confirmed with cultivation methods and biochemical tests. Then, bacteria were detected using PFGE typing and the results were compared with the results of antibiotic resistance. Results: The results showed that all isolates had multiple resistance. The highest sensitivity was observed for tobramycin (52%), gentamicin (36%) and moropenem (32%).The results of this study showed that A. baumannii strains isolated from Shahrekord hospitals were in seven different genetic patterns that two of these patterns were sporadic and the genetic patterns were different in each hospital. Conclusion: Although variations among strains of Acinetobacter baumannii were observed by using PFGE in Shahrekord, but no epidemic strain was detected among them. In terms of resistance to commonly used antibiotics were also different patterns.