بررسی ضرورت پوشانیدن گل ها در گرده افشانی کنترل شده سیب با ردیابی آلل های ریزماهواره در نتاج تلاقی ارقام گلدن ’ اسموتی ‘ ×شفیع آبادی

Authors

  • حاج نجاری, حسن
  • زمانی, ذبیح الله
  • طلایی, علیرضا
  • فتاحی مقدم, محمد رضا
  • گاردینر, سو
Abstract:

A population of offspring from a cross between ‘Golden Smoothee’ × ‘Shafi Abadi’ apple which was developed by two methods of controlled pollination (with and without covering after controlled pollination) was used to investigate the inheritance of microsatellite alleles and the necessity of covering in controlled pollination of apple. DNA was extracted from 60 seedlings (30 from each method) as well as corresponding parents and the probable source of unwanted pollen. Four microsatellite loci (CH03d12, CH03d07, CH04a12 and CH03c07) which were polymorphic among parents were selected and their florescent primers were prepared. DNA amplification was carried out using different colored florescent primers, and alleles size were determined using ABI377 automatic gene sequencer and Gene Scan Software version 2.0 according to internal standards. Results showed that all seedlings shared one allele at each locus with each parent indicating their hybrid and true to type nature. There were no differences between two methods of controlled pollination in terms of unwanted pollination and there were no off type seedling originating from unwanted pollen source. Allele distribution among the progenies showed their co-dominant mode of inheritance, and no significant difference with Mendelian co-dominant ratio (1:1:1:1) was observed using chi square (x2) test. These results showed that there was no need for covering after controlled pollination of apple at least for less sensitive cases such as cultivar breeding which takes lots of time and cost for controlled pollination of many flowers in a limited time span. Results also showed the importance and potential of microsatellite loci in detecting parent-offspring relationship prior to inheritance study of characters and alleles, bulk segregation analysis, gene and linkage map and historical reconstruction of fruit tree pedigree.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

اثر بربرین در تنظیم آستروسیتهای Gfap+ ناحیه هیپوکمپ موشهای صحرایی دیابتی شده با استرپتوزوتوسین

Background: Diabetes mellitus increases the risk of central nervous system (CNS) disorders such as stroke, seizures, dementia, and cognitive impairment. Berberine, a natural isoquinolne alkaloid, is reported to exhibit beneficial effect in various neurodegenerative and neuropsychiatric disorders. Moreover astrocytes are proving critical for normal CNS function, and alterations in their activity...

full text

اثر بربرین در تنظیم آستروسیتهای Gfap+ ناحیه هیپوکمپ موشهای صحرایی دیابتی شده با استرپتوزوتوسین

Background: Diabetes mellitus increases the risk of central nervous system (CNS) disorders such as stroke, seizures, dementia, and cognitive impairment. Berberine, a natural isoquinolne alkaloid, is reported to exhibit beneficial effect in various neurodegenerative and neuropsychiatric disorders. Moreover astrocytes are proving critical for normal CNS function, and alterations in their activity...

full text

انتخاب بهترین رقم گرده زا برای برخی از ارقام تجاری سیب ایرانی

اغلب ارقام سیب خودنابارورگزارش شدهاند، به طوری که انتخاب و معرفی ارقام سازگار برای تولید محصول مناسب از اهمیت ویژهای برخوردار میباشد. این پژوهش به منظور انتخاب مناسبترین رقم گردهزا بر دو رقم سیب تجاری ایرانی گلاب کهنز و شفیع آبادی که به دلیل زودرسی و عطر و طعم خاصی که دارند مورد توجه میباشند، انجام گرفت. در این آزمایش تاثیر 5 رقم گردهزای گلاب صحنه، گلاب کهنز، شفیع آبادی، نایون ارنگه و خورسیجان ...

full text

ارزیابی جمعیت های سیب حاصل از تلاقی کنترل شده ارقام ایرانی و خارجی و ارتباط بین صفات رویشی و زودباردهی

به منظور بررسی ارتباط بین برخی خصوصیات مورفولوژیکی، فنولوژیکی و زود باردهی در طول چندین سال متفاوت رشدی، پژوهشی روی جمعیت های سیب حاصل از تلاقی کنترل شده انجام گرفت. در این آزمایش برخی صفات کمی و کیفی در بیش از 400 نتاج حاصل از چهار ترکیب تلاقی سیب های ارقام گلاب و شفیع آبادی به عنوان والدین پدری و ارقام رداسپار و گلدن اسموتی به عنوان والدین مادری، اندازه گیری شدند. پارامترهای آماری مانند ضریب ...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 13  issue 48

pages  1- 15

publication date 2009-07

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

No Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023