بررسی ارتباط فاکتور چسبنده babA2 با cagA و vacAs1 در بیماری های گاسترودئودنال ناشی از هلیکوباکتر پایلوری

Background and purpose: cagA, vacAs1 and babA2 in Helicobacter pylori are important virulence factors associated with gastrointestinal diseases. The prevalence of these genes and their association with gastrointestinal disease is different around the world. Materials and methods: In a cross-sectional study, 1500 patients with gastroduodenal disorders were enrolled. They attended Imam Khomeini Hospital in Kermanshah 2013. Biopsy specimens were obtained from the antrum and corpus of stomach. The specimens were inoculated onto selective medium for 3-5 days. The suspected colonies of Helicobacter pylori were identified by morphology, biochemical tests and presence of ureC gene. The frequencies of cagA ،vacAs1 and babA2 genes were surveyed using PCR. Results: The frequencies of cagA, vacAs1 and babA2 genes were 89%, 58.3%, and 68%, respectively. There was no significant correlation between cagA, vacAs1 and babA2 with gastric and peptic ulcers. The frequency of triple-positive strains of cagA, vacAs1 and babA2 was 40.3%. Also, babA2 with cagA and vacAs1a were found in 66% and 43%, respectively. Conclusion: Triple-positive strains in H. pylori isolates were more frequent in patients with peptic ulcer compared to those of the gastritis but this difference was not significant. However, further investigations are required to clarify these results.