بررسی اثر عصاره هیدروالکلی گیاه علف چشمه (Nasturtium officinale) بر تغییرات بافتی و هورمونی تخمدان صدمه دیده با دوکسوروبیسین در موش‌های صحرایی

Background and Objectives: One of the side effects of chemotherapy is infertility and the use of Nasturtium Officinalis causes the anticancer molecules to be increased. This study was conducted to determine the effect of hydroalcoholic extract of Nasturtium Officinalis on ovarian changes after toxicity by doxorubicin in rats. Materials and Methods: In this experimental study, thirty-six mature, female Wistar rats were randomized into six equal groups including groups, 1, 2, and 3 which received distilled water, 25 and 75 mg/kg of hydroalcoholic extracts of Nasturtium officinale daily for 21 days, respectively. Group 4 received only one single injection of doxorubicin 10 mg/kg (IP) and groups 5 and 6 received doxorubicin and 25 and 75 mg/kg of the extracts daily for 21 days, respectively. The Luteinizing hormone (LH) and follicle-stimulating hormone (FSH), estrogen, progesterone and ovarian histological changes and the ovaries weights were assessed at the end of the study. Data were analyzed using one-way ANOVA followed by Tukey's multiple comparisons test. Results: The mean (mean &plusmn; SD) of FSH in the second and third groups were 0.21&plusmn;0.05 and 0.23&plusmn;0.01 respectively, that significantly increased compared to the fourth group 0.11&plusmn;0.01 (p<0.05). The mean of primary follicle in the fifth and sixth groups were 4.32&plusmn;1.31 and 5.41&plusmn;1.43, respectively, that didn't show a significant increase compared to the fourth group 3.01&plusmn;1.12, (p>0.05). The mean of graafian follicles in the fifth and sixth groups signifantly increased compared to the fourth group (p<0.05). Conclusion: The hydroalcoholic extract of Nasturtium Officinalis with antioxidant properties have efficacy on ovarian follicle to improve oogenesis following toxicity by doxorubicin in rats. Key words: Nasturtium officinalis, Doxorubicin, Ovary, Follicle, Hormone, Rat Funding: This research was funded by Yasuj University of Medical Sciences. Conflict of interest: None declared. Ethical approval: The Ethics Committee of Yasuj University of Medical Sciences approved the study. How to cite this article: Mohammadi J, Safari F, Rad P, Delaviz H. The Effect of Hydroalcoholic Extract of Nasturtium Officinalis on Ovarian Hormonal and Histological Changes after Toxicity by Doxorubicin in Rats. J RafsanjanUniv Med Sci 2016; 14(11): 953-64. [Farsi]