ایجاد لاین‌های تراریخت برای مطالعۀ نشو و نمای مغز در (Tribolium castaneum (Coleoptera: Tenebrionidae
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Abstract:
در حال حاضر، انتقال ژرم لاین در حشرات روشی گسترده برای تجزیه و تحلیل عملکرد ژن و توسعۀ گونه‌های اصلاح‌شدۀ ژنتیکی است. اطلاعات زیادی دربارۀ نشو و نمای قسمت‌هایی از مغز جنین حشرات موجود نیست. به این منظور، چندین لاین تراریخت T. castaneum به هدف تعیین نقش برخی ژن‌های درگیر در نشو و نمای مغز ایجاد گردید. توالی کامل ژنوم T. castaneumمشخص شده است و روش‌هایی نیز برای ایجاد حشرات تراریخت این گونه ارائه شده است. در این پژوهش از عنصر متحرک Bac piggy به عنوان ناقل بهT. castaneum استفاده شد. پلاسمید دهنده حاوی ناحیۀ ژنی مورد نظر، به جنین استرین چشم‌سفید (Tc vermilion-deficient) تزریق گردید. لاین جهش‌یافتۀ چشم‌سفید، دارای آسیب در ژن vermilionاست، اما از طرفی پلاسمید دهندۀ مورد نظر دارای ژن vermilionTc و تحت پروموتر 3xP3 است که سوسک‌های تراریخت را قادر به بیان ژن مذکور و در نتیجه ایجاد رنگ تیرۀ چشم می‌کند. در این مطالعه، لاین‌های تراریخت حاوی نواحی تنظیمی برخیژن‌های مهم در نشو و نمای مغز ایجاد شدند. این لاین‌ها به منظور ردیابی رشد نئورو بلاست‌ها از ابتدای رشد تا ساختار کاملاً رشدیافته در مغز حشرۀ تراریخت، ساخته شدند. به‌علاوه، این لاین‌ها امکان تشخیص ژن‌ها و مطالعات عملکردیشان را در مغز T. castaneum فراهم خواهند آورد.
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Journal title
volume 46 issue 2
pages 207- 218
publication date 2015-09-23
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