القا‌ی تومور آلوژنیک زیر جلدی گلایوما با سل لاین GL26 در Balb/c
Authors
Abstract:
Background and Objective: Glioma is the most common primary brain tumor. Despite many advances in treatment, all patients die within 6 to 18 months after diagnosis. In the cases of glioma, the immune system is suppressed in a local fashion. Therefore, unveiling the cellular and molecular mechanisms involved, with the aim of obtaining an appropriate new treatment is a priority. Designing an appropriate animal model is necessary before any clinical trials. Material & methods: In this study, we prepared fifteen 6-8 week-old female mice (Balb/C) from the Pasteur institute, Tehran,and also selected the mouse glioma cell line GL26 to induce a allogeneic subcutaneous tumor. After culturing the cell and anesthetization of the mice, we injected different cell doses into distinct groups of mice. Sterile PBS was injected into the control group. Animal behavior and clinical symptoms were regularly followed and recorded, and after tumor induction, it was surgically removed and evaluated in terms of macroscopic and microscopic characteristics. Results: The tumor was induced more quickly with higher number of GL26 cells in mice. Atrophy and weakness was observed in the affected animals. In macroscopic examination, the tumor was relatively large, thick and full of blood. Moreover, in microscopic examination, cell proliferation, mitosis, abundant vessels, and tumor necrosis were observed. Conclusion: Regarding the limitations of a glioma syngeneic animal model, establishment of an allogeneic subcutaneous model, allows an easy evaluation of the size and volume of the tumor, without a requirement for sacrificing the animal. This model has the potential to provide opportunities for research on some immunological parameters, the testing of new therapeutic agents, and new discoveries in basic research, concerning glioma, for the first time in Iran.
similar resources
القا ی تومور آلوژنیک زیر جلدی گلایوما با سل لاین gl۲۶ در balb/c
چکیده زمینه و هدف: گلایوما شایع ترین تومور اولیه ی مغز انسان است. با وجود پیشرفت های بسیاری در درمان، تمام بیماران 6 تا 18 ماه پس از تشخیص می میرند. در گلایوما سیستم ایمنی در محل تومورسرکوب می شود .بنابراین نیاز به پژوهش در مکانیزم های سلولی و مولکولی به منظور انتخاب روش درمانی تازه و اثر بخش از جمله ایمونوتراپی یک ضرورت است. لذا طراحی مدل حیوانی مناسب قبل از هرگونه کار آزمایی بالینی برای رسیدن...
full textاثر بربرین در تنظیم آستروسیتهای Gfap+ ناحیه هیپوکمپ موشهای صحرایی دیابتی شده با استرپتوزوتوسین
Background: Diabetes mellitus increases the risk of central nervous system (CNS) disorders such as stroke, seizures, dementia, and cognitive impairment. Berberine, a natural isoquinolne alkaloid, is reported to exhibit beneficial effect in various neurodegenerative and neuropsychiatric disorders. Moreover astrocytes are proving critical for normal CNS function, and alterations in their activity...
full textاثر بربرین در تنظیم آستروسیتهای Gfap+ ناحیه هیپوکمپ موشهای صحرایی دیابتی شده با استرپتوزوتوسین
Background: Diabetes mellitus increases the risk of central nervous system (CNS) disorders such as stroke, seizures, dementia, and cognitive impairment. Berberine, a natural isoquinolne alkaloid, is reported to exhibit beneficial effect in various neurodegenerative and neuropsychiatric disorders. Moreover astrocytes are proving critical for normal CNS function, and alterations in their activity...
full textگزارش یک مورد تظاهر غیرمعمول سل جلدی
Introduction: Because of the resurgence of tuberculosis in the world, cutaneous tuberculosis remains a clinical and diagnostic problem. Lupus vulgaris is the most common type of cutaneous tuberculosis. Case Report: Herein we present a 35-year-old male with multiple skin lesions in the face and neck since several years ago. Unfortunately due to unscientific manipulation, clinical appearance ...
full textفارماکوکینتیک فلورفنیکل در گوساله‌ها متعاقب یک تزریق زیر جلدی
فلورفنیکل یک آنتی بیوتیک وسیع الطیف و مشابه کلرامفنیکل است که مصرف آن در حیوانات مولد غذا تایید شده است. این مطالعه بخشی از یک پروژه بزرگتر است که با استفاده از مدلسازی فارماکوکینتیک– فارماکودینامیک برای ارزیابی داروهای ضدمیکروبی در پنومونی گوساله‌ها انجام شد. 10 رأCس گوساله سالم، داروی فلورفنیکل را بصورت یک تزریق زیر جلدی با دوز 40 میلیگرم بازای کیلوگرم وزن دریافت کردند و در زمانهای مشخص (0- ...
full textMy Resources
Journal title
volume 20 issue 78
pages 13- 22
publication date 2012-03
By following a journal you will be notified via email when a new issue of this journal is published.
No Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023