identification of toxic shock syndrome toxin-1 (tsst-1) gene in staphylococcus aureus isolated from bovine mastitis milk
Authors
abstract
staphylococcus aureus is a major causative pathogen of clinical and subclinical mastitis of dairy domestic ruminants. this agent produces a variety of extracellular toxins and virulence factors including toxic shock syndrome toxin-1 (tsst-1) which is the major cause of toxic shock syndrome (tss). in this study 58 s. aureus isolates obtained from 9 dairy herds in east and west azerbaijan provinces of iran. the tested isolates were identified on the basis of the cultural and biochemical properties as well as by amplification of the aroa gene, specific to s. aureus. isolates were also analyzed for the presence of the tsst-1 encoding gene (tst) using polymerase chain reaction (pcr). tst gene 350 bp was detected in 9 (15.5%) of the total number of 58 isolates. the present study revealed that the pcr amplification of the aroa gene could be used as a powerful tool for identification of s. aureus from the cases of bovine mastitis. results also showed that the strain of s. aureus which caused mastitis can potentially produce staphylococcal toxic shock syndrome toxin-1. overall, our results suggest that it is of special importance to follow the presence of tsst-1 producing s. aureus in foodstuffs, especially for protecting the consumers from toxic shock syndrome.
similar resources
Identification of Toxic Shock Syndrome Toxin-1 (TSST-1) gene in Staphylococcus aureus isolated from bovine mastitis milk
Staphylococcus aureus is a major causative pathogen of clinical and subclinical mastitis of dairy domestic ruminants. This agent produces a variety of extracellular toxins and virulence factors including Toxic Shock Syndrome Toxin-1 (TSST-1) which is the major cause of Toxic Shock Syndrome (TSS). In this study 58 S. aureus isolates obtained from 9 dairy herds in East and West Azerbaijan provinc...
full textdye labelled monoclonal antibody assay for detection of toxic shock syndrome toxin -1 from staphylococcus aureus
objective : the aim of study was to develop a rapid assay, dye labelled monoclonal antibody assay (dlmaa), using non-radioactive organic synthetic dyes for identification of toxic shock syndrome toxin-1 (tsst-1) producing strains of staphylococcus aureus. materials and methods: the assay protocol required only two simple steps; addition of tsst-1 antigen to a nitrocellulose membrane and then ad...
full textToxic shock syndrome toxin 1 (TSST-1) production by staphylococci isolated from goats and presence of specific antibodies to TSST-1 in serum and milk.
The ability of staphylococcal strains isolated from different anatomical sites in 133 healthy goats to produce toxic shock syndrome toxin 1 (TSST-1) and the presence of antibodies to this toxin in serum and milk were studied. The enzyme-linked immunosorbent assay method was used to detect both the toxin and the presence of antibodies. Of a total of 342 staphylococcal strains studied, 86 (25.2%)...
full textSurvey of accessory gene regulator (agr) groups and TSST-1 encoding gene (tst) in Staphylococcus aureus isolated from ewes with mastitis in the northwest of Iran
Accessory gene regulator (agr locus) is a global regulator of many virulence gene expressions inStaphylococcus aureus. Four interference classes related to genetic polymorphisms in the agr locus have sofar been described. In the present study, the agr locus specificity groups were studied within a total of 43 S.aureus isolates which were isolated from ewes with mastitis in three regions in the ...
full textPrevalence of toxic shock syndrome toxin 1 (TSST-1)-producing strains of Staphylococcus aureus and antibody to TSST-1 among healthy Japanese women.
Many cases of neonatal toxic shock syndrome (TSS)-like exanthematous disease but few cases of menstrual TSS (mTSS) have been reported in Japan. We determined the prevalence of mucosal colonization with Staphylococcus aureus and of positive antibodies to TSS toxin 1 (TSST-1) among 209 healthy Japanese women in Tokyo. S. aureus isolates from mucosal sites were characterized with respect to TSST-1...
full textThe Prevalence of Toxin Shock Syndrome oxin ( TSST-1) Producing Clinical Isolates of Staphylococcus aureus StrainsIsolated from Shohada Hospital in Tabriz, Iran
Abstract Background and objectives: Staphylococcus aureus is one of the most important etiological agents of hospital and community acquired infections. The enterotoxins and toxin shock syndrome toxin (TSST-1) are among the most common virulent determinants of this bacterium. They are also well-known for their super-antigenic properties. The incidence of TSST-1 producing strains is also very al...
full textMy Resources
Save resource for easier access later
Journal title:
archives of razi institutePublisher: razi vaccine & serum research institute (rvsri)
ISSN 0365-3439
volume 68
issue 1 2016
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023