characterization of the salmonella isolates from backyard chickens in north of iran, by serotyping, multiplex pcr and antibiotic resistance analysis

Authors

m.h. bozorgmehri fard

m. hassanzadeh

s.h. emaddi chashni

s. mirzaie

abstract

the present study was designed to investigate the prevalence of salmonella species, their molecular characterization and antibiotic resistance in backyard chickens. a total of 1125 samples were collected from backyard chickens in two consequent samplings. in the first part, samples were included of 820 poor recently hatched chicks, hatching residuals, egg shells in the nest floor, cloacal swabs and fresh litter droppings in the villages which located in north of iran. secondly, 305 samples were taken from newly hatched-chicks which fertile eggs were obtained from the rural chickens of those regions and incubated in laboratory incubator. of 1125 samples tested, 27 (2.4 %) salmonella were isolated that identified as serovars of salmonella enteritidis (55.5 %), salmonella typhimurium (22.2 %), salmonella hadar (14.8 %) and salmonella infantis (7.4 %). except the traditional serotyping that was performed for all isolates, salmonella typhimurium and salmonella enteritidis isolates were characterized by using multiplex pcr for further identification. all of six typhimurium serovars were positive for rfbj, fljb, inva and flic genes. in the case of enteritidis serovars, polymerase chain reaction generated amplification products for spv, sefa and random sequence (specific for the genus salmonella) in all of fifteen samples. most of the salmonella isolates in this study were sensitive to norfloxacin.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Characterization of the Salmonella Isolates from Backyard Chickens in North of Iran, by Serotyping, Multiplex PCR and Antibiotic Resistance Analysis

The present study was designed to investigate the prevalence of Salmonella species, their molecular characterization and antibiotic resistance in backyard chickens. A total of 1125 samples were collected from backyard chickens in two consequent samplings. In the first part, samples were included of 820 poor recently hatched chicks, hatching residuals, egg shells in the nest floor, cloacal swabs...

full text

Molecular identification of Salmonella Infantis isolated from backyard chickens and detection of their resistance genes by PCR

This study aims at molecular identification of Salmonella Infantis isolated from backyard chickens and the detection of their antibiotic resistance genes. A total of 46 Salmonella-suspected samples isolated from backyard chickens of northern Iran were collected. Serotyping was done by the traditional method and then confirmed by PCR. Antimicrobial susceptibility of the isolates against 13 antim...

full text

the analysis of the role of the speech acts theory in translating and dubbing hollywood films

از محوری ترین اثراتی که یک فیلم سینمایی ایجاد می کند دیالوگ هایی است که هنرپیش گان فیلم میگویند. به زعم یک فیلم ساز, یک شیوه متأثر نمودن مخاطب از اثر منظوره نیروی گفتارهای گوینده, مثل نیروی عاطفی, ترس آور, غم انگیز, هیجان انگیز و غیره, است. این مطالعه به بررسی این مسأله مبادرت کرده است که آیا نیروی فراگفتاری هنرپیش گان به مثابه ی اعمال گفتاری در پنج فیلم هالیوودی در نسخه های دوبله شده باز تولید...

15 صفحه اول

Molecular identification of Salmonella Infantis isolated from backyard chickens and detection of their resistance genesby PCR.

This study aims at molecular identification of Salmonella Infantis isolated from backyard chickens and the detection of their antibiotic resistance genes. A total of 46 Salmonella-suspected samples isolated from backyard chickens of northern Iran were collected. Serotyping was done by the traditional method and then confirmed by PCR. Antimicrobial susceptibility of the isolates against 13 antim...

full text

the stady and analysis of rice agroclimatology in lenjan

the west of esfahan province, iran, is one of the most important agricultural areas throughout the country due to the climate variability and life-giving water of zayanderood river. rice is one of the major and economic crops in this area. the most important climatic elements in agricultural activities which should be considered include temperature, relative humidity, precipitation and wind. so...

15 صفحه اول

Characterization of Salmonella isolates from poultry sources in Iran

BACKGROUND: Salmonellosis is one of the most importantzoonotic diseases throughout the world. OBJECTIVES: Thepurpose of this study was to characterize a large collection ofSalmonella isolates from different poultry sources in Iran.METHODS: A total of 123 Salmonella isolates from differentpoultry sources were subjected to drug susceptibility test,hemolysin production, motility test, and plasmid ...

full text

My Resources

Save resource for easier access later


Journal title:
archives of razi institute

Publisher: razi vaccine & serum research institute (rvsri)

ISSN 0365-3439

volume 64

issue 2 2016

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023