exploring the protective effect of ascorbic acid and aqueous extract of spirulina platensis on methotrexate-induced lipid peroxidation

this study was designed to explore the protective effects of ascorbic acid and water extract of spirulina platensis (blue green algae) on methotrexate-induced lipid peroxidation using goat liver as the lipid source. this in vitro evaluation was done by measuring the malondialdehyde, 4-hydroxy-2-nonenal, reduced glutathione and nitric oxide content of tissue homogenates. the study reveals the lipid peroxidation induction capacity of methotrexate, the anti-peroxidative potential of ascorbic acid and aqueous extract of s. platensis on methotrexate-induced lipid peroxidation.